(May be wrong place, please direct me where I should post) Introduction

Testing and Analytics
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Hello, my name is Jordan and I’m an Account Executive at Delta Verde Labs in Phoenix, Arizona.
We hold the first ISO Certification in the state and are expanding our operations to provide education and testing to anyone nationwide.

I am a new member here, so please point me in the right direction on where this post should go if I have put it in the wrong discussion!

Have great day F-4200 and thank you for your endless supply of knowledge!

Jordan

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which cannabinoids are you testing for?
what else can you ID?

do you have a “delta 10” standard?

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We are testing for 16 Cannabinoids currently: CBDA, CBDV, CBDA, CBGA, CBA, CBD, THCV, THCVA, CBN, CBNA, D-8 THC, D-9 THC, CBL, CBC, THCA, CBCA.

As far as Delta-10 standard we cannot get standards to calibrate Delta 10 so there is no way to validate it in our data at the moment.

And to answer which else we can ID, can you specify a little bit more?

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Caymen has them

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pretty sure Caymen has one…

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Do the COAs have QR codes, and product photos? Those are my two biggest needs, after accurate testing. How much do yall charge for product cannabinoid profile?

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Thank you guys very much for sharing the Cayman resource, it is something that we are absolutely going to look into here in the near future. After discussing it internally we aren’t locked in on what the cost would be to get our matrix spikes isolated properly, as well as we would want to establish quality control methods and SOP’s to reliably put our name behind that data. So now that we have a direction and some insight on Delta-10, we’ll check out these suggested resources we will be digging more into now!

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Yes we sure do, QR codes as well as product photos.

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I am buddies with one of the original founders of this company. They come well recommended from me.

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Can you or anyone else explain to me the purpose of matrix spikes? If you have a validated method for flower, distillate or isolate why would you need to perform a routine matrix spike every batch? Shouldn’t a blank, LCS (I would go as far to say this could be omitted for a properly validated method), and CCV be all that is required to establish a properly functioning system? It is my understanding that labs generally only do one matrix spike sample and a duplicate matrix spike for each batch placed on the instrument. When it comes to edibles shouldn’t you really be doing a matrix spike on every sample due to the variability in matrix components from different types of edibles?

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Shouldn’t a blank, LCS (I would go as far to say this could be omitted for a properly validated method), and CCV be all that is required to establish a properly functioning system?

  • A matrix spike program will not only allow you to calculate an RPD for the specific matrix but also you will be able to see if your extraction method creates any cross chemical reactions with other cannabinoids. Matrix spikes also helps with further method validation and R&D. Because of the extra molecules that the matrix adds to your solvent (terpenes, lipids), you may discover you need to increase your solvent volume to avoid total saturation of the solvent. That causes suppression of your spiked analyte concentration due to not being able to extract it from your matrix. Finally, it gives you a better idea of how the matrix reacts with your column. Do molecules in your matrix bind to active sites on the column that analytes normally bind to? Thus resulting in a suppression of concentration in your chromatograms. A matrix spike will allow you to identify any miscalculations that arise inside the column. This is called the “matrix effect” and can be accounted for and therefore compensated by using a post column infusion. (Compensation of matrix effects by postcolumn infusion of a monitor substance in multiresidue analysis with LC-MS/MS - PubMed) These are validations that an LCS cannot perform and in my opinion a matrix spike is required for method validation.

When it comes to edibles shouldn’t you really be doing a matrix spike on every sample due to the variability in matrix components from different types of edibles?

  • Yes for good science it would be ideal to do a matrix spike on every edible sample but from an economic point of view it’s way more efficient to spike a parent sample and apply that data to all edibles in your batch.
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Today’s problem is “what’s in the flask?”…

Hemp derived CBD isolate that has been deliberately isomerized.

I can get some useful information on how well the reaction is proceeding, and when to stop using (say) an SRI-GC with an FID. Assuming standards, I can quantitate CBD, D8,D9,& CBN, but how much further can you get me?

How can I know that this “d8” I purchased off the inter web, or made in-house or at home Is actually safe to consume?

Will your LC/MS.MS come with a library that might identify unknowns, can you make guesses based on partial matches? What would that actually take?

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I agree with most of this but all of these things should be evaluated during method validation. During the validation to evaluate accuracy you would spike a matrix at different levels and see if your percent recovery is within the acceptance criteria. I just don’t think there is any point in performing matrix spikes during routine analysis after proper method validation unless you perform a matrix spike on every single sample.

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Can you tell “fake” shatter from the real McCoy?
It’s not a minor issue…

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The Cayman standard is only an analytical standard, which is nice for confirmation and estimation of concentration, but they don’t offer a certified reference material which is required per your ISO 17025 accreditation. Would be nice to find a delta-10 CRM.

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exactly. I was going down the same route and figured the same.