Review on Cannabis Tissue Culture

[Ralf]

From Could anyone help me hunt down Starburst OG? :

@anon64373531

Holy shit. $500 per culture? That gets you how many explants?

Tissue culture is on my bucket list of skills and it just jumped higher. Especially synthetic seed tech.

Same here. And in the future we may not have to mess with synthetic seeds as a means of long-term cryopreservation:

Cryopreservation of Shoot Tips of Elite Cultivars of Cannabis sativa L. by...

Also, this is a good paper in relation to maintaining long-term genetic fidelity though TC regeneration:

---

Viruses, Viroids, and Cannabis: A Brief Primer

@Ralf

TC can take care of viruses and viroids. If someone has cuts they love, TC can do wonders for its fidelity, too, if you can find a TC lab that does DNA demethylation.

Thwarting a Potential Cannabis Catastrophe With Science

@FicklePickle

At around 3000 a cut. And then it’s still not guaranteed to work

@Ralf

Yeah, def, not cheap. And takes a long time. I haven’t contacted Front Range, but if they’re at $3,000, I hope they would offer a partial refund if they didn’t succeed. I’m looking for labs in the US that offer this service to price compare. There def is a market for this, esp at a lower price point, so I hope to see more labs in the future.

@BananaBannabis

We use someone that does node culture for around $500

Meristem TC is more expensive and takes longer. It is my understanding meristem regen should “clean out more” then typical node culture. However node culture still has a high probability of cleaning out contaminants.

@emdub27

Front Range Bio can’t even keep their own stock clean. Specifically at the Salinas CA location. Stay far away.