BM folks who extract with CO2 are few and far between. Akin to a 
.
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I have a very deep love for psychedelics and what they have done for me along the way. Sadly I donāt have any tek to share with you as I would always just eat them. But I do wish you the very best on your adventure and hope thereās a day where I find myself trying mushroom extract!
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All right about the extraction, thanks! it starts to makes sense, also thanks for the following table. I think I start to slowly get it about converting psilocybin into psilocin, as both are soluble in different solvents, thus converting all into psilocin it makes life easier for purification. However there is still something I do not fully get: According to your table, Psilocin is more soluble in ether than water and should then partitioned into that non-acquous phase. The ether phase should contain psilocin thenā¦
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That table and the paper it came from doesnt specify the ph of the water used. @canucknotinuk
It was in another paper I read that both psilocybin and psilocin are soluble in dilute acetic acid. Psilocin would rather stay in the aqueous portion with a ph of 8. At the same time, psilocin rapidly degrades when dissolved in a basic solution so the ether wash needs to be done quickly.
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Sorry, im getting shit all mixed up. This is incorrect the psilocin should jump to the ether.
This is not applicable to the method I was trying to describe, I read this in a psilocybin isolation procedure. psilocybin would rather stay in the aqueous phase, psilocin would rather jump to the ether.
Gartzā paper describes isolation of psilocin and states that when the ph of the aqueous solution is brought up to 8, psilocin is more soluble in ether. Psilocin is already unstable in solution and begins to rapidly degrade at a ph of 7 or higher so you need to work very quickly to avoid unnecessary degradation.
@canucknotinuk
Since fresh psilocybe mushrooms tend to only have trace amounts of psilocin and contain mostly psilocybin you could perform an initial extraction on the mushrooms with chloroform or heptane prior to extracting with acetic acid instead of going the route of LLE. If you do strip with an organic solvent id recommend doing it on the biomass and then fully evaporating the solvent from the mushrooms. If you use chloroform and donāt remove it all prior to extracting with acetic acid the chloroform is going to fuck with your ether pulls by causing an emulsion to form.
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Looking for an extraction chemist in Vancouver to help a client of ours for their psilocybin extraction project. If you are competent and live around, leave me a message. (Not sure if it was the best place to leave this posting - Please guide me if a wrong place).
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super critical?
do you have any info on co2 extraction? or would ultrasonic be the best method for this?
Iāve got a eden 20L I might be Able to play aroundā¦ but its been sitting for awhile so it hasnāt seen much use in awhile
Iāve got no information for CO2 extraction of mushrooms. As far as extraction efficiency CO2 would be far superior for initial extraction than any other solvent due to the fact that supercritical co2 can penetrate cell walls and extract their contents.
CO2 used non selectively will probably just extract anything there is to extract. Good for separation from biomass but probably not for isolation of the active compounds from the resulting mixture.
But selectively extracting with CO2 would probably give the best results you could ask for. Hard for anyone but a well equipped lab to test that one tho.
Iāve got to assume that the well equipped labs that are doing this RnD are not sharing their data.
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yeah might be something ill just have to play around with and see if it has any benefits over other extraction methodsā¦
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What machine are you running?
well as far as co2 goes weāve got an eden lab 20L
and then thereās a hand full of rotos and short paths
and for closed loops thereās a variety of systems. Ive got a Bizzy bee but could probably piece together a small test system from random parts ive got around the shopā¦ parts from many different systems weāve had over the yearsā¦
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I gotta ask: which Vancouver?
Bumping in case itās near Portlandā¦
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Think itās BC, so youāre looking at about five hours on the road.
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Iāve done many things but 10 hours a day of commute hasnāt been one of them fortunately. Best of luck to candidates that make the cut!
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I think the Oregon boys are trying to get something rolling. Chime in.
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Yes, Vancouver BC. But happy to collaborate remotely as well, in case you can help us.
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Iām not a doctor, you need a doctor 
.
That being said, itād be really cool to setup a time to talk on the phone just to be sure sometime when you arenāt swamped.
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Hey everyone. Iām new to this whole thing, but have been pouring through forums, web pages, papers etc. Iām enjoying the research, but not having a chemistry background (but willing to learn) itās a bit overwhelming. Ultimately, what Iām looking for is a way to get reasonably accurate dosages for microdosing (and occasional trips). Iām hoping to end up with something that has a decent shelf life so I donāt need to make a batch from dried material more than once a month.
I was planning on going with the 200 proof ethanol tek, as it seemed the most straight forward, but it seems like the science doesnāt support this approach.
Iām wondering if thereās a āhobbyistā variant to the acetic acid tek that doesnāt require ammonium hydroxide, ether, or nitrogen; yet, will meet my objectives.
Also want to know if an ultrasonic cleaner would be useful, vs the lab quality devices. Most āconsumerā cleaners are 40Khz, though Iāve found one on Amazon that also has a 20Khz setting. No idea what frequency is the best.
TIA.
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