Love reading all your posts, it gets me excited to give it a go at some point. Do you have any info on where or how to test your biomass for psilocybin/psilocin content?
4 Likes
Not right now I don’t, I assume that next year oregon will probably have somewhere to test product. I think you would probably need HPLC to test your biomass.
2 Likes
I have read that a gc and hplc can do it. I have both at my work and would love to learn more about how to do it from someone with experience.
2 Likes
Best I could do is help you to produce a reasonably pure sample of psilocin. I’ve read that psilocin can be phosphorylated back into psilocybin. Maybe you can produce a standard for both psilocin and psilocybin this way?
That paper I linked is pretty rad, take a look.
Hard to say what purity we can get, but theyve got opposite solubility characteristics so starting from a reasonably pure sample is worth a try.
3 Likes
I’ve read of antisolvent crystallization of psilocybin using acetone but I’ve got no experience with this process.
The paper I linked mentions isopropyl alcohol as being effective as an antisolvent for psilocybin as well.
1 Like
I’m down… didn;t realize how many of us were in Eugene!
Been in canna in Wa for years, Hemp in Oregon for the last 3 or so and moving toward psili and have access to some analytical equipment, a basic lab set up (in an analytical facility) and am down to meet up and team up.
5 Likes
Basic question… how does one go about steam treating? Something as simple as placing the harvest over boiling water? How long should they be treated? Thanks!
2 Likes
I’d like more info on this too @ScoobyDoobie Scoob
3 Likes
I started off my first attempt about 2.5 months ago with the ultimate grow kit from midwest. I’ve been extremely pleased with the results and shocked how easy it was after following their great directions. For this noob, having it all spelled out, with all the materials needed, it was definitely worth the money. That said, now that I know what it takes, If I had to replace the kit I’d probably just put it together myself and order the jars.
1 Like
I’m a little confused on this one. In the acetic acid approach its seems the ether is used to extract p-cin, but in this approach you’re washing the “undesirables” out with the ether? Is this because the ethanol/water prevents the p-cin from going to the ether, or its just not enough to matter for consumption?
Just simply exposing the mushrooms to an atmosphere of steam for about 5 mins right after you pick them is sufficient to denature the enzymes. It doesn’t work as well if you try to steam mushrooms that have already been dried. Steam treating mushrooms will always dephosphorylate at least some of the psilocybin so if you wanted to avoid any conversion to help assist with separation you could instead macerate the fresh picked mushrooms with a high shear homogenizer in ethanol to bust open the cell walls, denature the enzymes, and produce an initial extract to work with.
I’m not sure if methanol has the same effect on the enzymes but I’d hazard a guess that it does.
Its just the high temperatures of the steam that densture the enzymes, not the steam itself. Steam just works well to transmit that heat. This is especially true when the mushrooms are still wet.
https://onlinelibrary.wiley.com/doi/abs/10.1002/dta.2950
This paper suggests that if you extract right after you pick your shrooms you can minimize the enzymatic degradation of the alkaloids and end up with a higher yield of the secondary tryptamines. Probably isn’t necessary because the enzymes will be destroyed but you can add a dessicant to the ethanol to absorb any water thats extracted from the mushrooms, since the enzymes require water to work.
8 Likes
Thanks for the info! I am working with my own freshies. I don’t have access to a high shear homogenizer, though I may pick up an ultrasonic cleaner and give that a go. At the very least, it sounds like the steam will do less conversion than without.
2 Likes
Not sure if simply immersing a flask in an ultrasonic bath would be sufficiently destructive to the tissues like an ultrasonic homogenizer would do. However, it will definitely help when extracting the actives from the fruits because agitation is still agitation.
I suggest grinding your mushrooms in a porcelain bowl like a mortar and pestle with a bit of clean sand to use as an abrasive and a bit of ethanol to initially break down ur mushrooms. Then dilute with more ethanol and soak to extract.
That paper I posted compares a lot of the different parameters of extraction. Its a very good resource.
3 Likes
This is definitely true as far as long term storage. And if you just want to eat the mushrooms and you’re goal is to prolong the shelf life of the actives instead of extracting, then you can just steam the mushrooms and dry them like normal. You denature the enzymes and increase the shelf life of the active compounds.
I remember reading 69ron’s posts on the nexus about this a while back. He claimed that steam treating the mushrooms made them have a faster onset of action due to the conversion to psilocin. Apparently there’s a subjective difference in effect between equivalent dosages of psilocin vs. psilocybin. Psilocin allegedly comes on stronger because it does not need to be dephosphorylated by your body so it is all absorbed at the same time. I dint know the validity of that claim but that 69ron guy was a very smart dude. I wish he would make an account on this forum, im sure he can respect the growth of publicly available knowledge that this website and its members host.
8 Likes
You should send him an invite!
1 Like
If I knew him personally I would, he got into a big argument with the administrators of the nexus over that whole oilahausca thing and he got himself banned. Haven’t seen hair or hide of him since like 2008.
1 Like
Oh man thats a bummer to hear!
Took a little while to get around to it but 
Oh how I hate the smell of the mushies. Paired with warm water 
But I weathered the storm 
Edit…just in time for the holiday (inlaws) family visits 
11 Likes
gummies coming up?
I decided to try and make some popsicles, I grabbed some fungi from a friend but I’m waiting on the tubes. Lemon tek → koolaid/sugar/sweet shit → freeze pop
7 Likes
I used a shit tone of citric acid…talk about make your ass pukerup…
8 Likes