/r/sporetraders as well. ITW (mentioned above) looks like they’ve been around some cool places.
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Whats the best extraction solvent for mushrooms?
Methanol?
"Use of three types of solvent (polar protic, polar aprotic & non polar) is recommended especially if you don’t know exactly what you are looking for.
It also depend on the column you will be using for the chromatography and mass spectroscopy.
Also in line with I Nyoman Pugeg Aryantha."
“Often solvents like ethyl acetate, acetone or methanol are often used. Concerning my experience, methanol often leads to the extraction of high amounts of hydrophilic substances like sugars whereas ethyl acetate and acetone are more lipophilic, but still can be used to extract a broad range of metabolites. Unfortunately fungi often produce huge amounts of fat which are easily soluble especially in ethyl acetate and acetone. Depending on the nature of the compound to be extracted you can also add acidic or basic modifiers.”
"I suggest you use three group of solvent i.e polar, polar aprotic and polar protic solvent to cover the all range of compounds
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Polar protic solvent (water, ethanol, methanol, acetic acid etc…)
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Polar aprotic solvent (dichloromethane, ethylacetate, acetone, acetonitryle etc…)
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Non polar solvent (benzene, hexane, chloroform etc…)
When using water, you may need to extract at higher temperature (be careful with non heat resistance compund)
Make sure the toxicity status of the solvent when developing product to be consumed"
Found it here from a 2014 forum, so take this with a grain of salt with regards to newer capabilities. https://www.researchgate.net/post/What-is-the-best-solvent-for-extracting-secondary-metabolite-from-endophytic-fungi
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Methanol isn’t bad, but acetic acid is better, then going for a standard acid/base workup.
Psilocybin is a zwitterion and hence does not separate well with an a/b, but psilocin can be extracted this way just fine.
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Wouldnt that be an issue and youd leave alot of psilocybin behind sir?
Of course that’s an issue, if you want to extract all of the actives, you’ll have to be ok with pulling some inactives. It really only matters how pure you want your stuff to be. If you’re after pure Psilocin, chemical synthesis should be much more attractive.
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So acetic acid with a/b workup is the most effective way to extract all active compounds in the mushroom? Are you against methonal?
Dude. Gummies and medieval times!
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We can do a grab bag special, reach in and take a few, after you eat them ill tell you the possible outcomes. 
or 
or 
or 
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Sounds like a fun night. 
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Acetic acid will extract both Psilocin and Psilocybin, as will Methanol. Both solvents will additionally extract some inactives based on their polarity.
Hmm. What do you think about DCM?
DM me if you need a consultation.
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That picture is moving on its own
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Makes you wonder what hemp fed bovine shit would produce
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Yea, but acetic acid is better choice between the two. Methanol co extracts more shit than acetic acid.
Isolation is kind of a pain if you aren’t in control of producing the biomass you’re using for extraction. In house analytics are a handy tool. If you can reliably produce biomass high in psilocybin with negligible quantities of psilocin then you can make your isolation a hell of a lot easier by initially extracting your biomass with chloroform since psilocybin is insoluble in chloroform.
The purpose would be to remove as much shit as you can prior to extraction. Psilocybin can be recrystalized in distilled water, the trick is to subject the solution to I pressure to superheat the water below its boiling point. Boiling water works too but its harder to work with and causes unnecessary degradation due to the amount of heat you’re exposing the psilocybin to, you can increase solubility while maintaining lower Temps by introducing pressure.
Acetic acid is nice because its makes conversion and extraction essentially one step. Youre gonna have to soak the biomass for hours because the compounds are contained in the tissues. I haven’t used ultrasonics for extraction yet but I plan to.
Another thing, if you have control of production of your mushrooms you should be steam treating your mushrooms right at harvest to inactivate the enzymes responsible for the blue brusing that results from the oxidation of psilocybin when the inner tissues are exposed to oxygen. This is the primary reason shrooms lose their potency so quickly, you can extend the shelf life of your actives by months just by inactivating the enzymes at harvest.
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When I do try ultrasonics I’m going to be using it during the chloroform soak, hopefully it busts the cell walls wide open to allow for a quicker acetic acid soak.
I feel like if I use ultrasound during the acetic acid soak ill probably defeat the main purpose of using acetic acid instead of methanol. I’ll probably extract anything methanol would extract in those circumstance.
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With it having pretty good solubility in water, that’s highly doubtful. It’s rather recrystalized from Alcohols.
Or you can just get rid of Psilocin during acid/base workup as it forms a water soluble phenolate salt.
I don’t get it, do you want more heat or less heat?
https://courses.lumenlearning.com/introchem/chapter/solubility-and-pressure/
For condensed phases (solids and liquids), the pressure dependence of solubility is typically weak and is usually neglected in practice.
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You always mention the chloroform as a wash step, is there any alternative solvent to use? heptane?
Super helpful post btw!!! 
How potent do you think your average mushroom extract is with psilosibin % sir? Methanol or AA tech…Since its usually 2% in bio for actives correct?
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Heptane would work too but chloroform is weakly polar so it’ll extract more garbage than an alkane would. Both would work but heptane won’t be as effective because it just doesn’t have the capacity to dissolve as broad a spectrum of compounds as chloroform. Thays why I recommend chloroform over an alkane.
I dephosphorylate to psilocin for the final product because thats how i know how to crystallize. I probably yield like .8% psilocin from the biomass. I dont have analytics so I csnt really tell what the purity is but I can get crystals and I know it’s psilocin.
The reason for my shit yields is because the lad I get biomass from doesnt test the biomass when they harvest so psilocybin and psilocin content tends to vary. The process I use is by no means an SOP If you’re looking for an SOP you should pay for a consult.
If you convert to psilocin, having biomass high in psilocybin allows you to initially soak your biomass with chloroform or an alkane.
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