Ive spent the better part of 6 months piecing together a lab for the goal of making high end concentrates - not distilling.
rotovap and auxiliary equipment are taken care of,
i found a 6kg panda for a steal of a deal recently, and as well found a -86C 40gal freezer in perfect condition that was practically given away.
im trying to work out the details for resonance times and temps and the practicality of it all.
I’ll spell out my proposed SOP and let you pick it apart.
1)1 kilo of biomass in 100 micron bier filter bags is placed in an 18L bucket with lid over night at room temperature with x amount of silica gel to desicate remaining water content.
2)removing silica gel, biomass in bucket are placed in freezer over night, with 190 proof ethanol next to it in the freezer
3)8L of ethanol is poured into the bucket without removing either ethanol or work bucket form the freezer
4)x saturation time
5)bucket is removed from freezer and bag of biomass is quickly placed in panda centrifuge where remaining ethanol is drained collected along with the ethanol remaining in saturation bucket.
6)5 Liters of crude solution at a time (remaining is placed in freezer to wait its turn) is filtered through 250mm büchner funnel with 20micron filter paper, and collected before immediately being processed through a 1-3 micron filter paper and finally a celite 545 filter on a fritted disc before going into the Rotovap, while i process the last of the crude/ethanol solution through filters that i placed in the freezer, and then rotovap for it too
my preocupation is with saturation time of ethanol and biomass, and how necesary is agitation of during saturation. My goal is to not have to use active carbon or t5 at all. my goal being, getting efficient yields without techniques that require post processing of coloration
part of why this has been a 6 month adventure is my budget jacketed reactors arent much of an option for me.
i do have a (about) 12 liter CLS for butane thats jacketd that ive never used that could also be an option forethanol biomass extraction (i guess a giant steel buchner funnel thats super tall and heavy and jacketed), but id like to avoid using dry ice at all costs.
i appreciate you help and im happy to share data as i get running