yep. Process was Herbolea user was in Zimbabwe iirc.
no liquor though.
Tried to help them out, but they had run out of trust and wtf would hire some @cyclopath they met on the internet?!?
@Ganjineer710 please don’t take offense @moronnabis I swear he is not deliberately trying you to piss you off.
No offense taken. I understand the skepticism. Lots of ghosts have flaunted proprietary technology behind NDAs and tease for attention here.
Yeah, the folks who tried selling me their “works for Tomatoes, so it should work for cannabis” back in 2014 or 15 where unintentionally isomerizing.
With lycopene that was a plus…
I do however get a glimmer of what you’re sending back from beyond the event horizon, and am absolutely willing to concede that they may simply not have been hitting the high notes correctly.
#fuckyeah!
Thanks…for update on the greater scope of ultrasonics.
I might be a little blinded but I don’t see any great future for the EAE.
I’m about 80% convinced that the enzymatic part of herbolea’s process is purely a MacGuffin for the suits who don’t know any better.
The product it produces is garbage, but at least the equipment is expensive and the yeields are shite!
If you place trim/flower/bubble hash in PROPER pH WATER you will almost instantaneously extract the cannabinoic acids as a solute. Is this a “thesis” or a “Hypothesis” you are putting forward. At the PROPER pH it is highly unlikely that your Enzyme works.
Please show us data concerning YOUR ENZYME activity data on test compounds at varying pH say 5-13. Capisce?
Please out line the pH values you think are relevant for your floating materials, and provide some conceptual framework for the ionization state of the carboxylic acid. I should remind you that THCA and CBDA are all very different in these regards (solubility). Please note reference above, if CBDA is highly soluble in 50% ethanol why would you add enzymes to have it float on top…show is some qualitative data of molecules in each fraction of your “thesis” SOP???
Can you even add biomass to the aqueous enzyme solution and stir it without knocking the trichomes off. Are you adding salt? I can not quite follow what you are doing…you have data that suggests THCA floats when dropped in water?? Please advise.
Now there is some data (Heads phenomena) that suggests a compartmentalization of Cannabinoic Acid storage in trichome capitate storage areas. If your enzyme thesis concerns mid pH ranges, it is possible that you could chew away at the capitate exterior surface and liberate a material that represents Cannabinoic Acids complexed with some additional compound (such as Palmitamide), and that the complexed material is not solvated and has a combined density such that it floats.
Whether or not it would ever be a profitable extraction method is moot, but just the demonstration of the floating complex would certainly reveal some mysteries of the in situ state of capitate storage areas.
I don’t have a MY enzyme because I’m not associated with Carbon Chemistry anymore. The only reason I commented is because my email is blowing up with comments on a post I made years ago. Notice the past tense framing used in my comment. This entire concept sat around for a long time while other products and processes were developed. IMO, there is no need for enzyme assisted extraction as I’m thoroughly in the “non-polar solvent covers the bases” camp.
Thanks, butane certainly does the job.
Regards,
Well it seems as if the subject of EAE has temporarily been beaten like a rented mule.
Wouldn’t it be easier to mod yeast to just make THCa?
Microbiology is where I come from and I remember reading about this being done nearly twenty years ago.
Literally vat farm your THCa and blast down your grows trim with hydrocarbons for the terps?
Would be a fun and arguably more profitable use for those brewtech unitanks certain ethanol extraction “designers” are fond of.
I’ve seen a few people who are much smarter than I do analysis of having yeast produce cannabinoids, and the economics don’t work out for CBD and THC.
If the plant only produced them at 0.1%, maybe. But easily getting 20%+ from tossing some seeds in a field makes biosynth not viable.
For trace ones that are rare/valuable, it could possibly make sense.
From what I understand, all of the companies chasing that particular tail are really hoping some rare cannabinoid turns out to cure cancer or something similar.
Sounds like they should let a brewer have at the problem.
That won’t overcome the cytotoxicity, lack of a specialized storage compartment, or low yields.
Solvent extracting 10kg biomass to get 1kg cannabinoids vs dewatering 1000l of culture media, then extracting 100kg of cells to get 1kg cannabinoids isn’t a “brewer” issue.
IMO.
No worries, we all have our opinions, but in my experience, it’s an awfully simple task to harvest the trub without even halting fermentation!
Besides, wouldn’t cultivation be a more appropriate comparison than extraction? Since that’s ultimately what it would be replacing (for bulk cannabinoid production).
Regardless, it’s all a heck of a lot more accessible than tailored enzymes.
It’s been a long time since the conversation I had with a group that was doing this, but I seem to remember that they said it was going to be 10,000 L of yeast per kilo of cannabinoids.
I probably should have said “That’s amazing and definitely going to make you tons of money” instead of something along the lines of “You’d have to pay for our services in advance if you want us to take it on.”
10 10bbl conicals is like, a moderately successful local microbrewery
Have at it…
Oh lol, Eurofin? I’ve got a deep in there, brb lmao
Given the math above, those 10 tanks would get you 333 grams of cannabinoids per batch.
Which math?
Lets say its the 1000L to 100kg of cells to 1kg cannabinoids,
At roughly 1000L per 10bbl conical, wouldn’t that be roughly 1kg per conical for a total of 10kg per batch? As opposed to your 333g per 10 conicals?
Lets take it a step further and assume that a primary fermentation is complete in roughly 10 days, and we’re re-pitching with top trub or just re-feeding/hydrating because we’re not actually brewing beer here, just yeast farming. So you could theoretically get 3 batches a month (though irl you’d be doing staggered batches most likely), for 30kg of cannabinoids, making it around 1kg of cannabinoids a day. I don’t know about you, but yeast are a hell of a lot easier to automate than cultivation sites.
I’m certain the numbers being thrown around are just that, imo, but my interest is piqued, I’ve got the gear at home already, and I’ve got a line coming in the mail.
