XAD-2? @NoHeatNoVacuum
3 Likes
That’s a great one, I’ve had a lot of great luck with some of the older Dowex products that have been replaced by several other new products. Just seeing the number of different fractions I can pull based on pH and polarity is amazing. I’m currently collecting fractions this way and rotovapping them down for analysis. One of my current pet projects.
3 Likes
This is like a fun homework assignment that might lead to nowhere.
https://twin.sci-hub.se/6830/61fe19c5d30adba1e4ade0b8ba6204f0/williams2010.pdf
3a mol — my guess is the resin is the whole situation…and no one knows what that resin is yet.
3 Likes
XAD is baller for seperation between the pentyls and the propyls…
5 Likes
Fun! how are you quantifying? I wonder if I could set something up with the fraction finder to try something like that out? Ill have to poke around for some medias. What solvents you using? I wish i was associated with a larger outfit to be able to afford cool research stuff… all these extra curricular purchases really leave me in the po’ house!
3 Likes
This tech (bids) would be awesome to implement but with current pricing of all fancy raisins it could be projected as the “overkill price” to implement on the big scale …
For the people on this forum who was laze or busy to do the R&D on this - simple SOP for color removal ,thc CBD separation and surly for encourage to start the new topic in here.
Mix your crude or distillate with heptane 1:1 ( for crude soline wash is needed)
Get 3A zeolite (in jar for the simplicity) and wash it with fresh heptane ( drain solvent out)
Combine your mixture and zeolite (fill till zeolite fully suspended)
Wait few hours .
Filter ( coffee filter is fine for this step)
Liquid portion will go back for the re run (if CBD/thc separation is desired)
Zeolite in you filtration funnel now loaded with the goodies.
Prepare second filtration funnel for color CRC STYLE 1”-3” of b80 will work, put loaded zeolite on top
Run fresh Heptane ( acid spiked if not decarb crude was used) to wash zeolite. Collected heptane would be almost white and it will wash out only 50%-60% of all available goodies but zeolite will hold up the thc molecules a little better so with this method it is easy to bring down % of thc in oil drastically
My personal record from almost 8% down to 1.2%
Don’t forget zeolite still loaded with cannabinoids needs to be washed with different solvent (toluene)
Is the second solvent of choice to remove cannabinoids but not the garbage.
This SOP was for the 1st grade students … lol
For the pros:
3A is 0.3 nm al the bulky molecules can’t feet
Zeolite exist in the form of powder (column loading)
Zeolite based ion exchange resin will give have a force to hold molecules (thc/CBD separation)
Pre-wetting resin with the right (top secret ) solution will activate protonation of nitrogen in functional groups allowing the adsorption of acid ( everybody remembering the hint about controlled decarboxilatio
of THCA to thc and living cbda in acidic form)
So if someone will start the new topic on zeolite and polymer resins I’ll will tell what would happen if someone stupid/curious will use zeolite/resins in packable distillation head.
Happy Hollidays !!!
14 Likes
Once I clean them up, usually just redissolve for HPLC, if it’s hard to tell I’ll use the LCMS. Right now I’m just mainly playing with pH right now, but I had some really cool results salting out fractions. I am also working my way through solvents. If it’s safe to use, I’ve tried it. I like adding a little acid or base to methanol/ethanol. A good amount of phenolic analysis from wine crosses right over, but unfortunately for the longest the wine guys were artistic not scientific.
1 Like
dumb idea number 427: Biologists approach to THC isomerization
- over-express cannabinoid synthase of choice
- bind to agarose beads and setup for chromatography
- run crude over column and play with pH
8 Likes
Lightly off topic… And maybe @Photon_noir already solved the color problems with the opaline.
But doing a multiple size stack of zeolite (3a to 5a or something?) In a CRC, would it have some potential benefits in color remediation? A few people seemed to point out it would help, but I’m curious if anyone’s thinking about using this on thc-a heavy extracts to just help color.
(Yes I know I should just do it and report back 
)
3 Likes
Rereading a tread i started some time back and trying to Remember the circumstances i think it works
Some detail in my
Hexane crude tread yet seems Reading @Termite his info
I should have atleast rinse the seives for higher yield
2 Likes
Is this remediating THC or THCa?
Both for you half decarboxilate the oil prior to start !! Hmmm 
2 Likes
Anything that can be made through an absorbent in the flask can be made through reflux first then ran through a wiper after.
You know I love d8, and I use to make it with absorbents in the flask. It’s not very scalable though.
I’d rather reflux in a reactor and then distill again after in a wiper.
Guaranteed to be quicker
8 Likes
Update to #427:
Skip the beads: Just express that engineered synthase on the outside of some handy critter in as fashion such as this https://jb.asm.org/content/jb/177/6/1470.full.pdf. then use the critter instead of beads…removes the need to purify the protein or attach it to beads.
media costs = feeding your bioreactor to grow more of whatever critter you chose.
edit: remember, the DE which is a primary component of many of your color remediation or filtration media was a critter at some point. just shortening the wait time…by moving some bits around.
bonus points if you harvest the bacteria in a centrifuge, wash them in-situ, then perform your binding and pH washes without leaving the fuge.
7 Likes
I’m firing this lil guy up shortly, it’s hooked up to an LC that I haven’t even booted up yet… one thing at a time
Send me samples to run we got our analytics dept running full steam ahead real shortly
Thanks to @anon6488101 da real MVP
8 Likes
@Ruwan one more reason to get that big fancy bioreactor from your homies back in Wisco… I miss fermentation so much
6 Likes
ELSD is not going to work well on CBD and THC. Linearity will be an issue.
If anyone has analytical needs, I got you!
8 Likes
Could you give the hint again on partial decarboxilation cause i can t find it for the love of god 
2 Likes
Sure ,thc and CBD are very similar -it’s hard to separate this 2 ,
the biggest difference (exploit) would be between acidic molecule of CBDA and fully decarboxylated THC (not THCA).
Zeolite ( many types of raisins ) is used as a very “sticky” stationary phase ,
principal used is not a size exclusion but rather force interaction between stationary phase ( you can’t elute (make it move ) the thc and CBD but CBDA is moving easy down the column + b80 will clean the color if loaded at the bottom .
Try pentane to see how easy it is ( but only around 20%-30% would come out rest would be still stuck to the column .
Second wash -with the toluene(my choice ) all cannabinoids would come out almost nothing ( 3%-5% CBD/thc) And the colored junk will stay stuck to zeolite and b80 - I usually not do the 3rd wash sending zeolite and powders to garbage can.
2 Likes