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Osmond, C.B., Daley, P.F., Badger, M.R., Lüttge, U. 1998. Chlorophyll fluorescence quenching during photosynthetic induction in leaves of Abutilon striatum Dicks. Infected with Abutilon Mosaic Virus, observed with a field-portable imaging system. Bot. Acta 111: 390-397.
We reduced this to a field-portable setup for plant disease studies, but it was early 90s, so there were some tech hurdles that have disappeared since…science marches on!
Found something interesting. I decarboxylated the THCa in a capillary tube and it took on a green glow, then roasted the pooled extract over a butane cigarette lighter and it takes on blue glow just like isomerized oil or short path heads.
I have used UV to see the layers in a separatory funnel. Nothing glows terribly brightly, but seeing layer boundaries is easier. I tend toward dark extracts, for personal use, so seeing layers with similar color is difficult…UV helps a lot.
In addition to 365 nm UV lights having the advantage of not emitting violet light, many objects will fluoresce strongest at 365 nm, as evidenced by absorption spectrum measurements.
The result is that 365 nm light may be better suited for applications where stronger fluorescence effects are desired. Coupled with the advantage of having a lower amount of visible, violet light emitted, 365 nm light can considered an optimal choice where performance is of paramount importance.
Your best bet is going to be buying a UV lamp capable for going from 265 nm (common wavelength in flash chromatography systems) to 305 nm. These wavelengths are used for TLC and can be helpful for LLE visibility.
DO NOT! Expose skin, eyes, pets, or anything that has living dna/cells when working with uvc. Uvc will seriously fubar your life and plants up.
I make custom uvc boxes for growers.
I made some spider mites dance in circles in a baggie while I exposed them to direct uvc light (in a ziplock on a leaf).
Was actually funny looking at them after.
100% yes. UV is no joke when you start playing around with light stronger than something like a simple counterfeit bill detector or pet stain detector. Eye protection is a must when using very strong sources of of UV. You wont see very many blind people making extract.
Maybe this is relevant for the CRC tech that’s going around. The green fluorescence that I usually see in extract that’s been partially processed is totally stopped by neutral pH activated alumina. Whatever it is, it has crazy high retention in my little micro column. Mobile phase 99 percent iso.
These vails have different strains of cannabis, 91% IPA a little NaOH, and some also have carbon…but the carbon doesn’t seem to matter. Anyway, hitting them with UV light made them fluoresce in totally different colors. The camera doesn’t pick it up the same as the human eye, but close enough.
I’ll tell ya one thing, Philips 930 T9 bulb emits lots of uv. Did 2hrs flower work under the lights. Shoulders, back and head tingles, and in replace my floor every other run from uv dry rot. The plastic gets super brittle.
The t12 has a double glass jacket, this traps most of the uv compared to the t9 style.
the ceramic metal halide one? I’ve heard the new mh have high uv but I haven’t found one with the spectrum posted that rivaled hps. I’ve been toying with getting the reptile uv lights. specifically a t5 54w 46" 10%uvb/30%uva german made bulb from zoo med/reptisun
Yes, I only run Philips cmh bulbs. I grew up in fla on the gulf of Mexico. I clearly remember getting tingly from the suns uv Ray’s. Almost the same effect I get when I’m in my veg or flower rooms under the hoods. Ppe is strongly recommended.