So today I had a very interesting hour long phone call with one of the leading CRISPR scientists in the world whos in Korea. Basically they asked me if I could do anything to a hemp plant what would it be?
Take a wild guess at what I asked them to do…
Change the length of the carbon tail to make THC P or CBD P
I also asked them about splicing terpene profiles from cannabis into hemp, they said they think that’ll be easier then changing the length of the tail
They said they could most likely do this if they can find a strain with that molecule in it as they can trace the gene responsible and isolate it.
I found in the paper on CBDP/THCP where they got the strain they confirmed had it
Its an Italian strain that I guess the government has over there
I wanted to see if anyone else here has hear of any other strains with THC P or CBD P in them
If I can pull this off and I can get them a strain with one of those molecules in it there plan is to make seeds and sell them (and guess whos the US distributor )
It won’t be an enzyme breaking the tail, it’ll have a different input compound utilizing the same enzymes that make CBGa, prenyl transferase(geranyltransferase) . Same with THCv. THCv starts with divarinolic acid, Thc starts with Olivetolic acid. So for THCp or CBDp, we would need to identify the compound responsible which should just be the 7 carbon analogue of olivetolic acid(5-heptylresorcinolic acid) and the biosynthetic process involved in making it. From the attached image, it just should be Octanoyl-CoA.
I told them as a chemist to break the tail without destroying the molecules is nearly impossible. So they asked of id heard of an enzyme that’ll do it, I told them there might be an enzyme that could break the tail but I’ve never heard of it.
Thats actually exactly what I told them
I told them the starting material is a 7 chain tail instead of a 5 chain tail. I even told them it was the same gene to make thcp as it is to make thc the only difference is that starting material and the length of the tail.
Conventional genetics is a far simpler solution. The gene(s) required are already IN the plant (species). Informatics of the style @silverstudent is doing, along with appropriate analytics should be able to ID the specific paralog responsible for assembling with the heptyl tail. Marker assisted selection and conventional breeding will then get it anywhere you want.