so just about every time i post a video testing cannabinoids, i get about 5 people asking if i can test psilocybin with the GC. I would love to start testing for this but a lot of the research is pointing me to testing with a GC/MS
Anyone looking into this already… thought maybe the forum with a bunch of people who like to trip and a number of members with testing abilities can start a discussion on this.
I actually know a dude I sort of fancy as a mushroom guru - let me see what his 2 cents are about this
Just shot him a message - I should be seeing him later today actually. No kid for the weekend sooooooooo…
So it’s done with hplc.
It can be done with hplc, but this is a test kit kind of like ehrkich or mandelin reagent
Color turns corresponding to the amount of alkaloids in the mushroom
Looks like it ignores psilocin content though
He wants to know if you have access to a UPLC - Said as far as he knows that is the most accurate way. He also said if you do to talk to him if you’d like. He knows his shit.
I can confirm UPLC’s viability for this application.
You can use a multitude of instrumentation, but for what instrument can you get a DEA exempt standard to calibrate with? Thats gonna be your goal.
Psilocybin is much too polar to be going through a GC. This sounds like a task for reverse-phase LC.
If you want to test for psilocybin on a GC, you’re gonna need to do derivatization.
Who makes a DEA exempt standard?
Your local mad scientist
I’ve been trying to figure out how to drop fats and waxes. This part may help:
Sample Preparation:
Optional: Pulverize the mushroom.
Soak in Methanol.
Optional: Heat MeOH/Mushroom mixture (@40 degrees Celsius max) for two hours.
Pour off MeOH into a clean beaker and filter. Repeat MeOH soak, if desired.
Optional Step: Add 10ml Acetone, and place in freezer for 30 minutes to freeze out the fats. Filter.
Evaporate to dryness in a beaker without heat.
I bet acetonitrile would work even better.
Or a centrifuge
Most quality scientific papers dealing with quantitation and ratios of psilocybe alkaloids include which LC method they use; type of column, eluent/gradient, flow rate.
No. Psilocybin exists as a zwitterion. It’s not going to be suitable for reversed-phase. Maybe if there is an ion-pairing reagent. Or run as anion exchange chromatography. But C18 reversed phase won’t cut it.
GC won’t cut it either - the heat from the injection port and oven are going to dephosphorylate the psilocybin. Gotta use HPLC
10ml of acetone to what amount of meoh
I typically extract with 40ml MeOH but you are correct: they don’t say.
While I don’t see why the zwitterionic nature of psilocybin would complicate quantitation by reversed phase LC, I wouldn’t be surprised if there are better stationary phases and LC modes to choose from.
