so just about every time i post a video testing cannabinoids, i get about 5 people asking if i can test psilocybin with the GC. I would love to start testing for this but a lot of the research is pointing me to testing with a GC/MS
Anyone looking into this already… thought maybe the forum with a bunch of people who like to trip and a number of members with testing abilities can start a discussion on this.
I’ve been trying to figure out how to drop fats and waxes. This part may help:
Optional: Pulverize the mushroom.
Soak in Methanol.
Optional: Heat MeOH/Mushroom mixture (@40 degrees Celsius max) for two hours.
Pour off MeOH into a clean beaker and filter. Repeat MeOH soak, if desired. Optional Step: Add 10ml Acetone, and place in freezer for 30 minutes to freeze out the fats. Filter.
Evaporate to dryness in a beaker without heat.
No. Psilocybin exists as a zwitterion. It’s not going to be suitable for reversed-phase. Maybe if there is an ion-pairing reagent. Or run as anion exchange chromatography. But C18 reversed phase won’t cut it.
GC won’t cut it either - the heat from the injection port and oven are going to dephosphorylate the psilocybin. Gotta use HPLC
While I don’t see why the zwitterionic nature of psilocybin would complicate quantitation by reversed phase LC, I wouldn’t be surprised if there are better stationary phases and LC modes to choose from.