Can vs Will…,
Some (not all) operators are still misidentifying it at CBC.
So often there will be a spurious claim of CBC.
to claim otherwise seems disingenuous.
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Because most HPLC do not use DAD
Shit I tried to give botanacor a sample of d10 so they could test for it and they wouldnt do it lmao
This is why I use @AlexSiegel with norcal analytical
He has d10 and d6a10a
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Say what? What are they using?!?
I’m not an expert on hplc detectors, but was under the impression that a diode array was about the cheapest detector for LC.
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@Kingofthekush420 yeah no (what a surprise)
i run an agilent 1260. the whole cbc/d10 thing is NOT resolved with a DAD.
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If that were true why are they misidentifying d10 as cbc?
@extractepic has talked about HPLC with DAD being able to tell the difference in the d10 thread.
DAD from what I’ve been told is newer tech
You don’t know how to run your machine then obviously
Funny @extractepic can use DAD and tell the difference but you cant
You have 40 years of experience too and he doesnt even have a degree xD
Shows how much that expensive education did for you
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Still a “can” vs “will” thing.
My understanding, as a user of third party labs, not as an analytical chemist, is that if using a “standard” method, developed before folks started deliberately or accidentally making d10, then an operator looking only at the single channel involved in the quantitation, on that diode array may not notice the difference on the other channels.
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Or, quite possibly, they’re just running one of the many column/gradient combos where those compounds don’t co-elute. They’re out there. They did not co elute with my old hplc method with restek raptor column
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Thank you! Was just about to ask for your opinion 
@Kingofthekush420 is correct about identifying CBC vs d10; besides a very slight difference in column retention time (depending on method), there are visible indicators in DAD spectrum that highlight differences between CBC and d10. @MagisterChemist nd @cyclopath both make great points. it is 100% a case of “can” vs “will”, AND it also is dependent on hplc method. Our variety of methods give us nice peak resolution for d10 and its stereoisomers.
p.s. I do have a degree 
, just not a masters or PhD (yet).
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lmfao my god you are cringey.
@extractepic yes, obviously its possible. we are talking about liquid chromatography after all.
@cyclopath hit the nail on the head. if youre running a standard cannabinoid method you wont be able to split those two. yes, you can buy a standard of d10 and create a method yourself. no, no one (except probably @extractepic) has done so.
whats one?
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I didn’t buy a standard, I created one (and confirmed structure though NMR). Additionally, you can now buy ISO/CRM standards for d10 (and stereoisomers) through Cayman Chemical. Which means I’m definitely not the only one who has developed methods for separation. All about doing thorough research and exploring every outlet in this frontier industry 
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I’m wandering over here…and need a clue or three.
thats badass.
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I’m happy to help if I can. Give me a day or two for research, I’m not familiar with that instrument yet. And DM what the separation goal is.
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I don’t have the exact gradient times anymore, but the method went from around 60:40 methanol:10mM ammonium formate water to 100% methanol and back down, and the column was raptor ARC-18. With that method they were resolved with the d10 coming out right after d8 and then the CBC comes out about 10 seconds later. Degraded product showed large off target peaks but never high CBC.
huh, interesting, i run (ran) that exact method(different spike), with that exact column! in an 1160 and a 1260, and you definitely dont get elution like that at all.
with those conditions, youd see d8 near d9, which are both well before the cbc/d10 mess.
huh. well, hopefully someone else can chime in whos run this method, its fairly standard.
I don’t know what you consider “well before”, but the retention times were something like as follows:
D9: 9 min
D8: 9.25 min
D10 (inferred identity, as no standards were available at this time): 9.5 min
CBC: 10 min
Basically equidistant between the D9 and CBC, and very close to D8. I don’t know why the results were different from yours. Maybe the gradient. It was a nonlinear gradient which I don’t have exact parameters for currently. I tested many samples of distillate with “mystery peaks”, but never ever had elevated CBC, instead always getting large peak right after D8.
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Thanks for that, gonna chat with the analytical lab we work with.
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