Squigs Separation References and Discussion

A lot of what is needed in the Cannabinoid world is to do with Separation and Chromatography.

There is a lot of science that has been done on these topics and I am sure that more info
can not hurt the cause.

So I hope you enjoy my quest to becoming a better Cannabis Chemist and please feel free
to comment on any papers or comments in this thread.

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Thin layer chromatography in the analysis of cannabis and its components and synthetic cannabinoids

Journal of Liquid Chromatography & RelatedTechnologies

Thin layer chromatography in the analysis ofcannabis and its components and syntheticcannabinoids.pdf (1.9 MB)

Use of descending thin layer chromatography for identification of cannabinoids

Use of descending thin layer chromatography for identification of cannabinoids.pdf (1016.9 KB)

Centrifugal partition chromatography’s new use: medical marijuana

NatureResearch

Centrifugal partition chromatography’s new use_ medical marijuana Nature.com.pdf (551.4 KB)

CPC DISTRIBUTION CHROMATOGRAPHY OF CANNABINOIDS US Patent US 2018/0036278 Al
Feb. 8, 2018

US2018036278A1_Original_document_20200628084106.pdf (5.1 MB)

Preparative Isolation of Cannabinoids from Cannabis sativa by Centrifugal Partition Chromatography

Journal of Liquid Chromatography & Related Technologies

Preparative Isolation of Cannabinoids from Cannabissativa by Centrifugal Partition Chromatography.pdf (272.7 KB)

A preliminary study of the analysis of Cannabis by supercritical fluid chromatography with atmospheric pressure chemical ionisation mass spectroscopic detection

A preliminary study of the analysis of Cannabis by supercritical fluid chromatography.pdf (4.2 MB)

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industrial evaporation chromatography ideal combination for cannabis processing

please do not take brand advice from papers I post.

industrial-evaporation-chromatography-ideal-combination-for-cannabis-processing.pdf (439.3 KB)

The SFC Isolation and Purification of Cannabinoids using Application Specific Stationary Phases
Under Optimised Conditions

The-SFC-Isolation-and-Purification-of-Cannaboids-using-Applications-Specific-Stationary-Phases-Under-Optimised-Conditions.pdf (666.1 KB)

Analytical Separation Science Volume 4 Chapter 3

Separations with a Liquid Stationary Phase: Countercurrent Chromatography or Centrifugal Partition Chromatography

Separations with a Liquid Stationary Phase.pdf (8.3 MB)

Analytical Separation Science Volume 1 Chapter 1
Basic HPLC Theory and Definitions: Retention, Thermodynamics, Selectivity, Zone Spreading, Kinetics, and Resolution

Basic HPLC Theory and Definitions.pdf (1.0 MB)

Analytical Separation Science Volume 1 Chapter 2
Basic LC Method Development and Optimization

Chapter 2 Basic LC Method Development and Optimization.pdf (582.9 KB)

Analytical Separation Science Volume 1 Chapter 3
Recent Advances in Column Technology

Recent Advances in Column Technology.pdf (605.5 KB)

Analytical Separation Science Volume 1 Chapter 4
Hydrophilic Interaction Liquid Chromatography

Hydrophilic Interaction Liquid Chromatography.pdf (2.3 MB)

Analytical Separation Science Volume 1 Chapter 5
LC–MS Interfaces

LC–MS Interfaces.pdf (2.3 MB)

Analytical Separation Science Volume 1 Chapter 6
LC–MS Applications in Environmental and Food Analysis

LC–MS Applications in Environmental and Food Analysis.pdf (1.7 MB)

High-performance affinity chromatography of proteins on non-porous polystyrene beads

this is the one I want to look into :)

High-performance affinity chromatography of proteins on non-porous polystyrene beads.pdf (475.4 KB)

Fully Crosslinked Poly(styrene-co-divinylbenzene)Microspheres by Precipitation Polymerization andTheir Superior Thermal Properties

IVE FOUND IT :)

Fully Crosslinked Poly(styrene-co-divinylbenzene)Microspheres by Precipitation Polymerization andTheir Superior Thermal Properties.pdf (272.0 KB)

Effect of various synthesis parameters on styrene–divinylbenzene copolymer properties

Effect of various synthesis parameters on styrene–divinylbenzene copolymer properties.pdf (1.8 MB)

Copolymerization of Styrene and p-Divinylbenzene. Initial Rates and Gel Points

 instead of using AIBN to do the radical initialization use benzoyl peroxide.

just think surfboards man.

Copolymerization of Styrene and p-Divinylbenzene. Initial Rates and Gel Points.pdf (945.1 KB)

Preparation method of polystyrene-divinylbenzene microspheres for bilirubin adsorption

https://patents.google.com/patent/CN101357963A/en

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Whoever found you and got you here I owe a beer :beer:

12 Likes

someone gave me some info here on isomerising CBD when a lot of the information was hidden
or not explained properly (on purpose of course).
I am like many stuck indoors career in flames due to covid and thought to repay this act and
keep my self busy.

so the person to thank is the one who was the first to show kindness to me here.
ill let them decide if they wish to dob them selves in or not :slight_smile:

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method for preparing divinylbenzene by diethylbenzene dehydrogenation

https://patents.google.com/patent/CN100418937C/en

this method uses a modified iron oxide catalyst having diethylbenzene (low temp heat transfer fluid)
passed through a tube at 500 to 650 over the catalyst.

another synthesis that can be used to make divinylbenzene and can be done in a glass quickfit
set is the one published on prepchem

Synthesis of p-divinylbenzene

Under N2, 248.3 g (1 mol) of 1,4-bis(2-acetaminoethyl)benzene, 11.2 g (100 mmol) of potassium tert.-butylate, 95% pure, 26.4 g (100 mmol) of 1,4,7,10,13,16-hexaoxacyclooctadecane (18-crown-6), 250 g of a mixture of isomeric phenoxydiphenyl ethers, 250 mg of tert.-butylcatechol and 250 mg of p-phenylenediamine are initially introduced into a distillation apparatus analogous to Example 1. An additional 250 mg of tert.-butylcatechol are placed in the distillation receiver. After a vacuum of 10 mbar has been applied, the temperature of the bottom is increased. The cleavage reaction starts at about 175° C. and is detectable by the appearance of the products in the still head. The reaction is completed in 1-2 h by slowly increasing the bottom temperature to 210° C. Finally, the vacuum is increased to 5 mbar for a short time. The distillate is taken up in 300 ml of ether and 200 ml of water, the aqueous phase is separated off, and the organic phase is washed once more with 200 ml of water, dried with sodium sulfate and the solvent is removed by distillation in vacuo. 213.2 g of residue contains 58.4% of p-divinylbenzene (96% of theory), 2.6% of 4-(2-acetaminoethyl)styrene and 37.3% of isomeric phenoxydiphenyl ethers. 111.0 g of p-divinylbenzene are obtained with a boiling point of 40°-41° C. at about 0.5 mbar after renewed distillation over a 20 cm Vigreux column. Yield 85% of theory. Purity 99.9%.

Literature source US04650910

Synthesis of p-divinylbenzene.pdf (484.6 KB)

3 Likes

Analytical Separation Science Volume 2
Part1 Special Liquid Chromatography Modes
Chapter 1 Chiral Liquid Chromatography: Recent Applications with Special Emphasis on the Enantioseparation of Amino Compounds

Chiral Liquid Chromatography Recent Applications with Special Emphasis on the Enantioseparation of Amino Compounds.pdf (126.8 KB)

Analytical Separation Science Volume 2
Part1 Special Liquid Chromatography Modes
Chapter 2 Chiral Separation of Some Classes of Pesticides by HPLC Method

Chiral Separation of Some Classes of Pesticidesby HPLC Method.pdf (920.3 KB)

Analytical Separation Science Volume 2
Part1 Special Liquid Chromatography Modes
Chapter 3 Micellar Liquid Chromatography: Fundamentals

Micellar Liquid Chromatography Fundamentals.pdf (534.2 KB)

Analytical Separation Science Volume 2
Part1 Special Liquid Chromatography Modes
Chapter 4 Micellar Liquid Chromatography: Method Developmentand Applications

Micellar Liquid Chromatography Method Developmentand Applications.pdf (641.0 KB)

Analytical Separation Science Volume 2
Part1 Special Liquid Chromatography Modes
Chapter 5 Affinity Chromatography

Chapter 5 Affinity Chromatography.pdf (492.0 KB)

Analytical Separation Science Volume 2
Part1 Special Liquid Chromatography Modes
Chapter 6 Immunoaffinity Chromatography: Advantages and Limitations

Immunoaffinity Chromatography Advantages andLimitations.pdf (832.6 KB)

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I’d rather focus on thanking a contributor of data so heavy to others.

Maybe free hosting or technology consultations for apps / custom solutions / websites. An area I control cost on.

I’m the nerd begging to re-learn the lab but have far larger priorities like providing for a family with my current skillset.

Your friend can cut in on a free hosting/ website deal for a year :kissing_closed_eyes:

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High Speed Countercurrent is another choice to do cannabinoids separation, like CBD purification, THC remediation , etc. We provide such kind of system . Here is our website for system info : http://www.fulltrum.com

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How much does your smaller systems cost @whatsthat?

ok ill put up some info on countercurrent separation :slight_smile:

it looks like a continuous extractor for pulling compounds out of water but on steroids :slight_smile:

I love how its so small for processing 1 to 2 liters of oil in a day.

question what does it cost to fix it when it brakes ?

thankyou

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I’m really impressed by the video on the site. You have put a lot of effort to communicate.

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KVM for my database to stay safe and permanent.

I will then over time continue to rape the paywall for more complete journals that can
be downloaded in there entirety as they were originally published on paper.

though I would rather have a cheap price for good than a free one for a year.

its the long haul that makes the difference.

though the HDD is large like in the terrabytes.

we can talk latter about this thankyou for the offer.

I am sure this can be made to be a ftp site for all to use.

2 Likes

Analytical Separation Science Volume 2
Part 2 Capillary Electromigration Techniques
Chapter 7 Capillary Electromigration Techniques: Capillary Electrophoresis

Capillary Electromigration Techniques.pdf (653.7 KB)

Analytical Separation Science Volume 2
Part 2 Capillary Electromigration Techniques
Chapter 8 Modern Injection Modes (Stacking) for CE

Chapter 8 Modern Injection Modes (Stacking) for CE.pdf (1.5 MB)

Analytical Separation Science Volume 2
Part 2 Capillary Electromigration Techniques
Chapter 9 Capillary Gel Electrophoresis

Chapter 9 Capillary Gel Electrophoresis.pdf (888.6 KB)

Analytical Separation Science Volume 2
Part 2 Capillary Electromigration Techniques
Chapter 10 Nonaqueous Capillary Electrophoresis

Chapter 10 Nonaqueous Capillary Electrophoresis.pdf (2.5 MB)

Analytical Separation Science Volume 2
Part 2 Capillary Electromigration Techniques
Chapter 11 Detectors in Capillary Electrophoresis

Chapter 11 Detectors in Capillary Electrophoresis.pdf (546.7 KB)

Analytical Separation Science Volume 2
Part 2 Capillary Electromigration Techniques
Chapter 12 Trends in CE ‐MS and Applications

Chapter 12 Trends in CE ‐MS and Applications.pdf (1.4 MB)

Analytical Separation Science Volume 2
Part 2 Capillary Electromigration Techniques
Chapter 13 Capillary Electrochromatography

Chapter 13 Capillary Electrochromatography.pdf (3.1 MB)

Analytical Separation Science Volume 2
Part 2 Capillary Electromigration Techniques
Chapter 14 Micellar Electrokinetic Chromatography

Chapter 14 Micellar Electrokinetic Chromatography.pdf (2.2 MB)

Analytical Separation Science Volume 2
Part 2 Capillary Electromigration Techniques
Chapter 15 Chip‐Based Capillary Electrophoresis

Chapter 15 Chip‐Based Capillary Electrophoresis.pdf (405.7 KB)

Analytical Separation Science Volume 2
Part 2 Capillary Electromigration Techniques
Chapter 16 Chiral Separations by Capillary Electrophoresis

Chapter 16 Chiral Separations by Capillary Electrophoresis.pdf (1.4 MB)

Analytical Separation Science Volume 3
Chapter 1 Gas Chromatography: Theory and Thermodynamics, and Selectivity

Chapter 1 Gas Chromatography Theory and Thermodynamics, and Selectivity.pdf (642.9 KB)

Analytical Separation Science Volume 3
Chapter 2 Basic Overview on Gas Chromatography Injectors

Chapter 2 Basic Overview on Gas Chromatography Injectors.pdf (1.9 MB)

thoughts … questions …

when we have separated our cannabinoids into fractions via RP Chromatography
how do we see what the fractions are with out using RP TLC or indicators like the beam test
or some kind of spectral analysis ?

would making the DANSYL derivative of the phenolic group in the cannabinoids make them
polar enough to go through normal TLC ?

then we could use black light and watch them fluores

https://future4200.com/uploads/short-url/ljchOzEHNAZ1RIySHPQG78KmyLX.pdf

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This should be moved to The Data Dump

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if so please keep it a separate thread in the datadump :slight_smile:

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I’ll copy paste those there :slightly_smiling_face:

??

cant the whole thread just be moved ?

I even drew a wizard for you
radagast none the less

Yes. It’s been moved to data dump.

1 Like