Hi Friends, SOS!!!
We are a new in-house analytics lab in Oregon. Currently running potency on GC-FID with OpenLabs CDS Chemstation Edition. Eventually will be getting an HPLC to test the acid forms. There are a few of us chemists here from different backgrounds who have run these instruments before, but limited experience on method development.
There’s a ton of information online about the acquisition method as far as instrument parameters…but limited information on the initial sample prep math and inputting this into OpenLabs (sample amount, multipliers, dilution factors). Especially when sample amount box is unitless (ppm, mg, ug…!!!)
We were wondering when we are designing sample prep methods for different matrices, what are the things that need to be considered when comparing against %ESTD? Our curve runs from 0.5-250 ppm. (Open to using internal standards).
-How many mg to weigh out in how many ml solvent. Accounting for density of MeOH?
-Need to figure out how to report in % for flower and concentrate but mg/g for our edibles, topicals, tinctures, bath salts, bath bombs, pills etc…
For example, I know we have:
~20% flower
~70-80% concentrate
3g gummy at 10, 20, 40 mg/g.
3 oz jar of 100:100 mg THC:CBD topicals
15 g bath salt serving 1:1
1:1 bath bomb
Several tincture ratios…
What approach would you take to figure out the best sample prep MATH? We build some sheets on excel to calculate mg/ml, ppm. We can figure out homogenization but tips here would also help.
If anyone has any methods to the madness (literally) please share!