Alrighty, you’ll need an accurate scale, plenty of vials and accurate liquid measuring devices.
NOTE: When using measuring devices, the minimum weight you can accurately measure is equal to 0.412000d. Where d is the smallest display value. If your scale has a 0.0001 readability the minimum weight you can accurately measure is 0.4120000.0001 = 0.082g = 82mg.
So when you weigh, place a large amount on the scale and calculate what you’re removing.
Now, you want to determine the highest concentration of cannabinoid per given carrier. So determine what concentrations you want to test 10 mg/ml, 20 mg/ml, 30 mg/ml, etc.
Then, measure out several ml (let’s say 10-100ml) whatever accuracy your instruments can deliver—Pipettes are a good investment—and multiply it by the concentration to determine what weight of isolate to add. I.e. for 100mg of carrier and 10mg/ml you’d add 100ml*10mg/ml or 1000mg.
Repeat for each concentration three (or more) times in three separate vials. Let sit.
Visually inspect to see what solutions don’t crystallize and which do. That will give you a lower concentration limit in which you can safely determine no crystallization.
Ways to improve your experiment:
mix the solutions at different concentrations in bulk—it will give you more consistency. Don’t make solutions at 100ml. If you’re going to do five vials per concentration, make 500ml and then measure it out appropriately.
(I know my numbers are ridiculously high for volume, adjust appropriately)
Store the solutions long term and at constant temperature, this is how pharma does stability studies that give you an expiration date. You can do several temperatures simultaneously, a set of vials at 10C, a set at 25C, etc
Use homogenization and compare with hand mixed, this will determine the effectiveness of homogenization.
Obviously do this with different carriers (solvents) to determine their relative effectiveness for the task.
Keep results well organized or you’ll regret it.
Use AT LEAST THREE replicates or your results are statistically insignificant and basically don’t means squat.