Review on Cannabis Tissue Culture

[Ralf]

A couple of posts from Haze And Sour seem on topic as well:

Haze And Sour

To take the Sour D project one step further, I have this project flow in mind: Identification of the ‘real’ Sour D cuts using whole genome sequencing based on comparison to Chemdog 91, AJ’s Sour D, and agreed upon Sour D trait identification @silverstudent suggested Detection of DNA methylation through tissue culture of the selected Sour D cuts and the Chemdawg 91 cut. (This step may be unnecessary because it’s probably safe to assume the ~27-30-year-old genetic age of the cuts has introdu…

Haze And Sour

Maybe. With enough depth of coverage you could probably work out how many individual mutations you were looking at. When I was last worried about somoclonal variation (I’m of the camp that doesn’t draw a line between tissue culture induced vs “natural” genomic/phenotypic changes) we didn’t have access to massive sequencing…so I have to admit I haven’t seen data on exactly the kinds/scope/range of mutations or their frequency. I could make the same guesses I would have 20 years ago, re: deleti…

Haze And Sour

Thanks for the info! This is a topic where I know what I don’t know is vastly greater than what I do know. So, I love that you’re on this site and willing to share your expertise I have to stop by and buy you a beer or twenty the next time I’m on the west coast . Is it your opinion that somaclonal variations and somatic mutations are the same (or have the same result) but are from different causes? With the former mainly caused by newly induced mutations from some TC processes, an…