You literally don’t need much of a sample for TLC. You get 10 mg, dissolve it in a ml of solvent and then spot it onto your TLC plate. In a separate beaker you have the mobile phase you think will separate your stuff. You put the plate in and it will either verify or null your hypothesis.
Ahhhh who’s a salty little guy? It’s okay bud you can get a cool tag one day to.
But until then why don’t ya read further into it and keep the salt to yourself? Kids on this forum man, rly taking it down a notch. So you can feel free to be ignored and not see my posts anymore on the further 5 more gradients and solvents and all the other input you happily soak up.
Mmhmm. Okay bud. You go on with your bad self. Your an expert. Clearly. You caught me I’m just copying the SOPs the machinery companies give me…? lmao kids these days. This is what happens when everyone gets a participation trophy people.
A chemistry degree is super helpful. For sure. And you may know more about specifically chromatography then me. Congrats. Never claimed to be an expert at it nor would I ever consult on it.
Again, im not an expert but you continue to prove that you’re pretty clueless with anything but aquiring equipment and you make some pretty fantastical claims as far as that goes. Lol.
I dont know what me being young has to do with what we’re discussing its not like im a fucking high schooler, i guess everyone reacts different to having their feelings/pride hurt.
You can use it to help develop a solvent system without consuming liters upon liters of mobile phase and Kgs of stationary phase doing trial and error on an actual column.
If you wanna work with c18, you could use RP-HPLC to help ascertain optimal solvent composition for separation, and transfer method to your actual preparative-c18 column
Generally hydrocarbon solvents like heptane are used in normal phase chromatography