So a couple runs ago, i tried throwing just 3 or 4 inncoulated grains into a sub bag instead of the usual 3lbs of grain.
It took an extra 2 weeks to innoculate but it yielded nearly as much as the others, maybe 10-15% less.
I realized you really dont need grain, you can just underhydrate your subtrate while pasturizing and then get it up to field capacity using innoculated liquid culture.
The bottle neck of anyones operation is grain if your going the traditional route because a huge pasturizer is much easier/cheaper to make then a huge sterilizer.
The LC to sub route requires ALOT more liquid culture then lc to grain to sub, but ive been theorizing ways to make 5 gallon or pickle barrel sized “sterile” liquid culture.
Any vessel u use, needs to have one way valves, a 0.2 micron air filter and a pressure guage on it.
I think you can first of all clean and scrub the inside of a 5 gallon bucket as best u can then let it sun dry. Pour in some 70% alcohol (about 200ml) and close the top with ur valves and guage mounted to the lid. Swish around the bucket as best u can to hit every inside wall. You could heat up the bucket slightly and pull vac on it to pull out and evaporate the alcohol from the inside, making it sterile.
Then u need to inject sterile water, they make 0.2micron absolute water filters that would allow u to add in water that is sterile basically on tap.
Once u add 4 gallons of water u can then transfer ur already finished liquid culture into the bucket. To keep the lines sterile, i would use 200ppm HOCL water to fill the transfer tubes b4 liquid culture goes thru it, i imagine youll only need about 5-10ml of solution to fill ur tranafer tube assuming a 1/4" line from ur lc jar to bucket. Since the 5-10ml of sterilizing liquid will get diluted into the 4 gallons of water, i imagine it wont be at a strong enough solution to hinder the growth of the mycelium later on.
Attach a peristaltic pump to the 5 gallon bucket with a t valve on it. When necessary u can run hocl solution thru the t valve to clean ur lines.
You can automate alot of this and once the 5 gallon buckets are prepped they can store until u need to fill them with liquid culture, 15,000ml should make about 100 bags if your doing my underhydrate sub route. If your doing grain, u should have enough to innoculste 1000 3lb grain bags.
A glass container that is see through might be beneficial so u can see the liquid culture progression but daily sample can be taken because it will always be on a one way valve, only ever letting liquid exit, not enter.
Pickle barrels can be attached to tractor sprayers and you can hit a couple of acres in less than a day, cover it up with pasturized manure and straw. Wait for the rain in 2 weeks and go picking!