Mycology -- Tricks of the Trade

Do you notice something about this pin set?
I’ll give you a hint it’s from a clone.

Notice how they all are in clusters?

That’s because that’s typically the best place to take a tissue sample from.

Find a nice mature cluster, one you would like to see all over the tub. Take the sample from the base, where all the stems meet.
You want to get a small piece from inside the stem. Very small.
Transfer to a petri dish, and in a couple of days do another transfer into a second petri dish… because you are most likely going to have contamination in the first dish.

All that is done in front of a flow hood…FYI

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