I’m currently using methanol and water as my mobile phases on a separatory hplc. I read all around that methanol and water do not form an azeotrope, but I am finding it hard to separate the two based on boiling (evaporative) points. Have access to both a rotovap and FFE, not much luck getting over 97% purity though. and my 3A molecular sieves aren’t really doing much. Possibly drawing in moisture from the environment(?), I’m going to switch to sealed kegs and post my findings.
If anyone has some tips or pointers on how to better purify my methanol I’d greatly appreciate it. I really need to recover pure methanol so i don’t have to change my method/gradient all the time. If you have any questions on my processes, don’t hesitate to ask.