Nope.
Your gauge may have in.Hg below zero, but above zero is in almost certainly in psi. As one cannot achieve 50in.Hg vacuum, those are positive pressures and are in psi.
Nor are you running “BHO” over your biomass.
When you cleaned the system, did that include your solvent tank? Did you find water in there?
When you used “new tanks” was ALL the solvent “new” or did you just ADD new solvent to the system?
When you created a “new gas mix”, how did you determine the ratio? Was this new blend the ONLY solvent in the system?
Define “cold”. Ambient is 59F and it feels cold to me atm. Certainly would not qualify as “cold” for the purposes of light hydrocarbon solvent extraction of cannabis.
…and you haven’t regenerated or replaced your molsieve over the course of these 30-40 runs?!?
Sounds like you need to play hot potato…