Got it. So your sample was ran through a c-18 column and your cut your fractions before the thca and cbc eluted?
3 Likes
Yes sir
3 Likes
15 Likes
We had an insurance audit keeping us busy so we have ours still sitting in solution. Can’t wait to see what we end up with!
3 Likes
I’m recovering the hexane from my cbda conversion run as well!
5 Likes
Here’s my go at the room temp ptsa on some cbda/cbd starting material.
Looks like all the cbd And Cbda is completely gone. We are left with a majority of d8 with the d9 shoulder. And then thca is clearly there and the peak in question after thca is what we assume is thca d8.
This had almost no post processing cleanup work done as it’s just for r&d and curiosity.
13 Likes
That’s a damn fine result for your first go at it!
3 Likes
Nice! Id see that being where D8THCA would come out. Good separation from D9. You could sure separate that peak from the other isomer junk early on, at least less crazy gradient work. This could work much better on flash than the neutrals im thinking.
3 Likes
This would be much much easier to separate. I’m running it again with my 35 minute test method to see if i can get that shoulder spaced off more so i can quantify the d8 and share some potency numbers.
7 Likes
And do you think that D8 neutral is coming from decarbed CBD already in the starting product or is that decarbing in the room temp conversion?
1 Like
I’m thinking both
3 Likes
For sure a little bit of both. This was fine with a mix of cbda and cbd. Using a cbda isolate would really make it easier to see how much is decarbing in the process. This could possibly be prevented To a degree from running the reaction below room temp.
3 Likes
Looks beautiful!
3 Likes
I’m I understanding this - it looks like you have lots of neutral delta 8 THC and very little of the two acids? Based on 220nm it might even be decieving and there is truly very little acid and a ton of neutral, presumably because the conditions lend themselves to significant decarb?
1 Like
Correct. This was done using a starting material that was about 35% cbda 50%cbd. it’s mostly decarbed but the thca was about 17% so I don’t think it really decarbed much during the conversion.
I personally just did this for fun and have no plan to further this for anything more than learning and gaining more knowledge on hands on experience and tracking variables. I’d love to give it a run on some cbda isolate at some point too.
2 Likes
I understand, it was just preliminary exploratory work and I’m not here to criticize. For what its worth, that coincides with my own strictly preliminary results from a while back (2017). I just recently gained access to some old data, and it looked like from CBDA I had slow/poor conversion at room temperature and could only speed it up with heat - and the heat plus acid lent itself to decarb and byproducts of which I can only speculate. I haven’t tried using the whole array of sulfonic acids, my suspicion is theyre all birds of a feather and I’ll just see similar limitations across the board that they require some heat, but not too much heat and cause some decarb and its a clumsy game of stopping the reaction at just the right time. We’ve all established that the ring closing (cyclization) of CBD to THC is exothermic, I think we should expect the same with CBDA to THCA however this time temperature is even more the enemy since you want to maintain the carboxylic acid, and the number of potential byproducts is greater.
I’ve said before that I think CBDA isn’t the best starting material for d8THCA-A, because of its structure can form more products than if you started from d9-THCA-A under the same conditions. Get at me if you wanna talk some more about it, maybe there’s something here we can work on.
6 Likes
@thesk8nmidget Collaboration with @eyeworm sounds excellent.
3 Likes
2103KCA0290.0702 - Arendis Labs - D8THCa.pdf (185.5 KB)
@kcalabs says 88.7% d8thca
Not too shabby, but still room to work.
19 Likes