ADH is still dehydrogenating alcohol…so the ENZYME is not being inhibited.
the conversion of methanol IS being inhibited, because the affinity for ethanol, and its concentration are higher.
Fomepizole is NOT an alcohol, so it IS actually inhibiting the enzyme from performing its task.
But you’re logic resistant, so whatever…
The enzyme is still breaking down the femepizol though which is why they BOTH work through competitive inhibition
Im not sure what youre trying to get at here lol
You wanna back that up with the chemical reaction?
There is no -OH to turn into an =O in
Yep. That’s obvious.
The difference between inhibiting the enzyme and inhibiting a specific (toxic) reaction by said enzyme.
Give him a minute to find a response on Google
By definition Competitive inhibition is :
Competitive inhibition is interruption of a chemical pathway owing to one chemical substance inhibiting the effect of another by competing with it for binding or bonding
It literally has to do with the binding site not the reaction itself
Yes dear. We get that you don’t understand.
I believe you’re in good company though, because the MD you cited above saying that
Is metabolized to
by ADH is is incorrect.
That’s simply not a trick ADH can perform.
That’s a CytP450 trick.
Yep, it competes really well for binding ADH.
Nope, it’s not able to react…which is one reason it binds so well, the conformation change upon reaction is how the binding site is cleared. No reaction => limited release.
I get that you probably didn’t know it was an MD that you were quoting, because you didn’t look at the primary source…but the simple fact that there is no -OH to be dehydrogenated should have clued you into the fact that fomepizol is NOT being metabolized by Alcohol Dehydrogenase.
Still doesn’t change the fact both work on Competitive Inhibition lol they might work in different way enzymatically but they prevent the break down of methanol in the same way by affinity for the receptor
Receptors and enzymes both have binding sites and specific affinities…they are not the same critter.
…although biology works such that such a beast (receptor that sends a signal AND catalyzes a chemical reaction on its primary ligand) can not be ruled out.
The difference is that both methanol and ethanol are substrates for ADH, fomepizole is not.
Calling the PRIMARY substrate (ethanol) an “inhibitor” works for explaining the mechanism, but from an enzyme-centric point of view makes little sense…enzyme is still doing exactly what it’s supposed to.
Back on topic.
Tell us about your extraction/dewax/magic dirt column.
Let me know I’m wrong characterizing them as “at home”.
Acetone for the win!
Acetone will immediately drop a solid amount of shit for a room temp filter down to 1 micron. This is important, as ive noticed this significantly speeds up the second cold filtration, as the precipitated fats seem to aggregate a bit better and the filter flows a lot smoother.
For my second case, id say that most solvents cannot hold a stick to the low temps and fast strip that occurs with acetone in a rotovap. As a big bonus, you can keep your oil carboxylic and avoid a decarb if you wish to re-x with pentane after.
Why people use ethanol or methanol is beyond my comprehension.
Acetone fumes tends to go boom much sooner.
Well, some level of safety is assumed, at winterization temps the vapor pressure is pretty low, at 40c in a rotovap and slight vacuum, it evaps and condenses really quick.
Gasket material considerations and other handling materials are more of a pressing concern than vapors, in my opinion. Isopropyl is fine with mason jar lids, but acetone eats the gasket so no bueno.
Absolutely agree. As the adage goes, "To assume makes an ‘ass’ out of ‘u’ an ‘me’ ", but I do it constantly lol. I have a poor projection habit of assuming everyone thinks about safety in the sense that I do, usually when I’m feeling confident @cyclopath pops in with some chime in for the noobs I hadn’t considered. Teamwork makes the dreamwork.
Small closed loop system using afs filters. Really just want to make my own carts. So yes at home lol. Plan is winterize via ethanol. Rotovap. Spd.
whew…
do you already have the rotovap and Spd?
…the folks I’m working with have sold 1.5 million carts on the OR market, and we have not yet bothered with distillation or winterization.
Both of those ARE currently on my to do list, but you can absolutely make carts that folks keep coming back for without the effort of going to distillate.
both the routes we use have been thoroughly explored around here, see Decarb under pressure to retain terps? and Please humor me. How are decarbed diamonds that much different from distillate? as starting points.
chances are you’ll find plenty of links to threads exploring “carts” linked below the first posts…and littered throughout the thread.
assuming you already have the rotovap and SPD, there is also a ton of information around here on how to optimize both processes.
this tells us essentially nothing about your process. If you’d actually like to explore
you’re gonna need to tell the rest of the class exactly what you’re doing.
times, temps, input material, grind (don’t be like this guy), solvent ratio, dewax parameters, etc…
even then folks will essentially be guessing…
care to post pictures of your current work?
Edit: SPD Without Winterizing?
Im really starting out with indoor trim off a greenbroz. Its packed into jacketed column and brought to cold temps with dry ice butane is also cold af. Goes out the column hit afs light filter. Then into collection pot. (I use dry ice pellets if youd like that much info) lol. Then vac off with a oven. Pretty standard cls lol. I then end up with product from white clear to orange to yellow. Like pictures i sent above.
I will then proceded to winterize with um filter through a buchner funnel. I will then shove it in the rotovap, then into the short path. Then into carts.
Why do you want to distill that?
Sure. Do you also add isopropyl alcohol?
Using a slurry changes the thermal transfer properties (and hence your extraction/dewax temps).
Had to google “AFS filter” to figure out what you were using.
Even then it’s not clear what your “CRC” actually consists of…(Light CRC Botanical Extraction Filters – AFS Filter no info on what’s in it…)
AFS Filters are a simple solution that allows for the use of prepackaged filters, which are available in different recipes depending on the application. AFS Ultra Clear Cartridge – BVV
Are you using N2 to push that “cold AF” butane?
Solvent to biomass ratio?
description doesn’t seem to include a dewax…but instead you consider using “cold af” solvent to be equivalent?!?
You don’t pick up as many fats and waxes running cold, but that’s not the same as picking them up then removing them (dewax or winterize)…which is what you implied with
You want folks to tell you if your product is “wax free” enough to skip winterizing, but even after multiple requests for the required information to assess that, you skip the required details
So we’re back to
you don’t seem to understand how to describe what you’re doing in a manner that allows everyone reading to follow your process.
Good luck getting worthwhile feedback…
I give up lol i had one question you anwsered it in the first 2 posts lmao.
