You are literal proof that you can’t fix stupidity, hubris or being gilb as a muhfuh
You’re arguing about something thats been proven just because youre butthurt you got burned by cloners bad clones
Please explain to me how WHEN DONE PROPERLY tissue culture will sometimes work to remove the virus and sometimes wont
That literally doesnt make sense
Lol mr ferrule youre calling the kettle black here
I never got burned by cloner having dirty clones. Youre wrong
How about you stop being rude and calling folks stupid just because I don’t agree with your blanket statement. Especially considering your only talking about what someone else told you, not your own first hand knowledge of the subject.
6 Likes
Meristem culture works like… 40-70% of the time.
The reason behind this, is because you need the plant to grow it’s Meristem faster than the virus can spread through the tissue. When a plant is sick… It’s harder to do this. TC IS FAR FROM BULLET PROOF.
10 Likes
I never bought a clone from cloner
If the industry want a to talk about what’s known and isn’t (many labs go against your supposition, they probably aren’t as smart as you) then someone from the TC industry should make those statements. Not the friend of someone who did it once and sells grape juice filters.
Don’t be late for your appointment at Boeing. Got some engineers there that need you to tell them about your buddy that makes airplanes
I cut the tube and fixed the ferrule and bought a new one like my employer wanted
Gonna have to use another one juice box boy
1 Like
Not as simple as just being “done correctly”. You have to be able to outgrow the viroid as @vortal noted.
8 Likes
Bbbb bbbbb bbbut he has a friend that did it
3 Likes
You’re dealing with a “race condition”, so sometimes it WILL fail.
These guys claim “always faster”, but then add that doing other stuff AS WELL works better.
Meristem culture is a plant tissue culture technique in which a plant’s fastest growing tissue is cultured, and it is very helpful to produce virus-free plants, as the rate of meristem growth is always higher than virus cell to cell transmission. The technique of virus-free plant production using meristem was initially developed in 1952, and at present, it is frequently used to eradicate plant viruses from many economically important crops. The meristem culture combined with heat, chemicals, and radiation is proven to be more effective in virus elimination in many plants.
https://www.sciencedirect.com/science/article/pii/B9780323907958000175#!
5 Likes
Sounds to me like you didnt only take the meristem
This is probably why my friend said the meristem is always virus free
I’m not doing any TC personally. Just trying to provide the info you had left out. I highly recommend anyone curious to watch the video I linked.
6 Likes
Im not either im just proving info on what a close friend of mine said whos had luck removing it
You mean it didnt void the cert!
Funny how you do exactly what everyone tells you to do after arguing with them
1 Like
Have you studied intra-cellular virus movement in plants?
We’ve been working on meristem culture for quite some time.
Meristem culture does work. I would not consider it infallible.
It will depend on the virus, and the specific cultivar used. Virus either encode their own movement proteins, or co-opt those of their hosts. Single amino-acid changes in those proteins can radically change transmission rates. Cellular Targets of Functional and Dysfunctional Mutants of Tobacco Mosaic Virus Movement Protein Fused to Green Fluorescent Protein - PMC
It’s been 25years since my last plant virology course, and 35 since I was doing tissue culture for a living. If the meristem was always virus free, or always divided faster than the virus could move between cells, then there would be no need for heat treatment or addition of antiviral compounds to increase the success rate.
16 Likes
Glibbers gonna glib
Thanks for the experienced opinion and not mentioning your friend one time.
1 Like
Spent time in a couple of labs that were studying movement proteins…so yeah, speaking from a relatively informed position. Even if my experience is somewhat dated 
4 Likes
14 posts were split to a new topic: Acetylation of the frontal lobe
So are there any places I can buy seed from that don’t have weed herpes?
At this point it doesn’t seem like it.
1 Like
Buy clones from reputable sellers with current viroid tests
Quarantine what you buy and test it again yourself.
4 Likes
From what I have read, getting the viroid through seeds is technically possible, but exceedingly rare. If a seed has it, you will get an obvious runt that doesnt live very long to transmit the viroid.
2 Likes