which is why you dosed your wife at 400mg?!?
if you’re going to use a bioassay as your only analytics, you need to calibrate yourself and a couple or three others. you need a supply of tested/known potency material, and folks that are willing to get too high every now and then. Your wife may or may not be willing to play along at this point.
make a tincture in MCT at 10, 50, or 100mg/ml. then calibrate your team. most folks have a fairly narrow range over which they are useful, and are not particularly accurate. eg I can detect about 50mg, can tell 80mg from 100mg or 120mg, and “top out” around 150-200mg. I’ve run into users that topped out at 10mg and even one who gets high on 0.1mg and is gone for days with anything above 1mg.
first step is to establish each users sweet spot. where they enjoy the high. then their threshold and “OD” points. once you have those, you can test them for repeatability. administer their doses without revealing the dosage. do it in triplicate, several days apart. go up or down 20% and see if they can tell you the difference. double or half the dose, and disqualify any who’s dynamic range doesn’t extend that far.
see: Organoleptics: In House QC?
or tell us where you are, and maybe someone can suggest