Curious if anyone has tried complexing a crude psilocybe extract with cyclodextrin.
I think if you could just mask the mushroom flavor it would be a pretty viable alternative to isolation. Does anyone have experience making cyclodextrin complexes?
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I make them all the time with cannabinoids. I’ll try some out on a crude extract… I’ve also got some other encapsulation thoughts for protection from uv or oxidation.
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I want to learn more about these. Are they made with extracted tryptamines?
I have tried about 14mg of the psilocin freebase and it was extremely strong in its effects. I can’t imagine 2x that! A true heroic dose indeed.
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They are the shits…
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It’s an extract, not a concentrate. Typically during testing I’ll see tinctures made with biomass. These are concentrated mushrooms, complete with fats and lipids, and a rather full spectrum profile (multiplied mushroom smell).
The extract adaptatabs are ONLY psilocin. No smell.
Only one I’ve come across in the lab.
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Fascinating. I would bet it’s synthetic. Hat’s off to the chemist(s) whoever they are.
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The extraction gets an award.
But the compounding to make a dissolvable pressy? Chef’s kiss
Nintendo, anyone?
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Extracted from biomass 
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Is chromatography involved? Your method?
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This was a great read everyone. Thank you all for your input and knowledge. I’m sad that I reached the end.
I have limited access to lab equipment and solvents. Paranoid about ordering a lot of this stuff and having it delivered to my door. I have zero extraction experience, but have learned sooo much. I have googled a shit ton of acronyms lol.
Based on my research I will try the following:
Using an ultrasonic tub @43khz use methanol to do the initial extraction. Followed by 70%ethanol then acetic acid. 3 runs total.
Use a Büchner funnel to clean each run. Then use TheSource Turbo vacuum evaporator. This has a heating element that gets around 120F, hoping that will dephosphorolate the PCB.
Finally suck up the “Goo” with a syringe and inject it into gummies before they solidify. Hopefully suspending the goo inside will prevent oxidation.
I know it’s crude, but I think it’s the safest/easiest/cheapest route for a novice.
that’s a good option for 1lb at a time if you get a 6L
yes.
eh. probably better to do a meoh/etoh blend if you’re going to switch from one solvent to the next.
keep it anhydrous, also acetic acid is hard to purge, avoid this imo
90 mins each.
dump the ultrasonic into a 5gal bucket with 70um bag first.
strain, press with a wine press maybe.
no need to purposely dephosphorylate and probably wont w/o acid at that temp. dephosphorylation happens around 150c with heat alone
“Goo” Should be mostly water soluble.
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Why not just neutralize the acetic acid?
mainly the issue of aqueous extraction of the phosphatases. and having to keep your temps low (impossible in a bath ultrasonic) to prevent dephosphorylation in presence of acid.
but, neutralize as in basifying? what are you adding that won’t be a contaminant in the end product? bc keep in mind we’re not doing lle in this scenario.
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Yes, using a base to neutralize an acid will usually lead to the formation of a salt. The concentration of the salt formed is going to be so low and even still I cant think of why that would ruin the extract.
Might hinder further refinement, but I don’t think it’s a huge issue for what he’s doing.
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Appreciate you and Franklin chiming in with great advice and options.
If I do a meoh wash first should I follow with distilled water? Or vice versa?
Fixed that for you. Stoichiometry is a bitch.
ETA: it may be a dissolved salt, but it will always form the ions in solution.
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just do 3 washes of alcohol, if you want to use ethanol use something high proof 190-200 for the very last wash
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I don’t think dilute acetic acid neutralizes your phosphatases. so even if you neutralize the acetic for the smell it’s not a good idea bc of the water content alone.
I’ve seen some C1D1 flammable solvent ultrasonic baths that have jacketing for cooling. This is mostly to re-condense solvent vapors/prevent odors but might be worth checking out. Also, they provide an inert atmosphere with nitrogen.
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what size are they? with scaling after a certain point you’re better off building a stainless system.
additionally, running cold with mushrooms uses an UNGODLY amount of solvent
doesn’t a lot of alcohol processing fall under C1D2?
edit: oh jeez the small ones are expensive AF. just build something stainless for that amount…
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