Does anyone know?
Theoretically using multi dimension column chromatography (combinations of columns & buffer systems) you should be able to separate pretty much anything outside of isotopes.
The issue here is economy of scale. Not all methods are cheap and not all methods will give you perfectly separated peaks. Thus you will run up against hits to your yield or a rapid increase in the cost per run.
In order to get a better answer from the experts on this forum it’s probably best to provide some specific information about the purification you’re looking to do. What’s the scale, what equipment do you have access to, what are you making as a final product? Stuff like that.