For clarity I have a limited understanding of the actual formation of a real bonafide empty liposome produced in lab/pharma setting. I am intending to extort the nature of the surfactant, lecithin, and the shear ability of the sonicator to break the cannabinoid molecule clusters down to a smaller size alongside PC molecules also being ripped apart and hoping they find and bind to each other in a way that encapsulates the now smaller CBD molecule in a total vesicle size <100nm and homogenously suspended/dispersed in the bulk of the carrier oil.
Wouldn’t the lipophilic cannabinoid just be bound in the bilayer without the inclusion of a polar aqeous
solution/emulsion to occupy the core? I’ve had thoughts on that though, in all intents leaving the hydrophillic core empty would be a waste
Liposomes are formed when the water and the lipo/lecithin mix is hydrated
Your easiest method to form liposomes is as follows
Dissolve your PC/cannabinoid/surfactant mix into an organic solvent such as ethanol
Dry down the solvent
Using a high shear homogenizer, inensovely mix the lecithin/oil blend while slowly adding water
You have formed crude liposomes, the high shear should be able to take them down to a few hundred NM
Use an ultrasonic homogenizer to size down your liposomes and then make sure to run it through a nano-filter to enaure no toxic metals contaminants exist!
That was the first observations I made, using extract.
Later I found out that the solubility actually depends on the MCT oil composition and also on the presence of waxes. The limit of the pure CBD Isolate in the pure MCT oil I use is ~33% At room temperature (22°C). It can be increased to ~47% using hemp waxes (maybe higher). It gets 5-6% lower in other kind of oil such as olive or hempseed. The more linoleic acid the less it is soluble. Thus tuning the MCT oil C8:C10 composition may also allow to increase the solubility.
Solublity apppears also a few % lower for CBG.
I have not tried to find the limit for CBN. It is at least as soluble as CBG.
I understand the necessity of the water now and it’s role in the creation of the liposome.
I am probably not equipped to handle flammable solvents or to perform vapor capture or however else all of that is done safely to maintain ownership of all 10 fingers, eye balls and the like.
Are there any cold emulsion techniques you’re familiar with or if it’s possible even with the necessary addition of the water, how about with an additional surfactant like quijalla or similar?
I believe I would now be thinking of an O/W/O emulsion…
The addition of the lecithin and the use of the ultrasonic processing is in theory supposed to yield a product that is mostly comprised of evenly dispersed, encapsulated molecules of CBD(hopefully closer to the lower nm range) that due to not only the nature of the encapsulation but the suspension in oil instead of water resists things like coalescence or precipitation and allows the use of higher than described concentration levels again that’s at least the theory haha
Thank you for pointing out the linoleic acid reducing the solubility as grapeseed oil is high(er?) in linoleic and oleic acids.
Thanks for the suggested reading, not me that needs those.
OP is aiming at 333mg/ml cannabinoids. All I’m saying is that adding water won’t help THAT goal.
Totally agree that the key to delivery is bio-availability not active concentration.
my point was simply that the concentration they are aiming at is unlikely to be achievable once they add 50% by weight water, and they should adjust their goals (1 part water: 1 part Oil: 1 part cannabinoids is still 333mg/ml) .
the concept that a 50mg/ml liposomal preparation may be more helpful that a 333mg/ml MCT based tincture absolutely needs stating, but its not me that needs educated on the subject dear.
