Place some crude under high pressure and compare the decarb rate next to an open atmosphere vessel 
@eyeworm it dosenāt stop it be it does considerably change the reaction rate
Thatās dope. The company I worked for is the earliest one which start enzymatic here in China , but mainly focus on pharmaceutical or nutritional molecules ,I had the chance to communicate or work with the best team in USA like Professor Arnold before she won noble prize ,the bill catalysis team from those BIG pharmaceutical companies . Our R&D team was trying to develop enzymes for cannabinol but probably no progress since everything related to cannabinol are banned. While we did hear companies in USA did great job already
thatās just because you havenāt gotten your solvent out yetā¦
in order to do that experiment ācorrectlyā we would need to measure the amount of work being done (heat energy applied). from here it seems above my pay grade.
I was asking for the logic. because I donāt understand how it would work.
I also donāt understand how to accurately measure/control the bits what seem to need measured/controlled to actually test your hypothesis.
Edit: mind you, Iām trying to catch up on my homework, and am a couple of hrs deep in Organoleptics: In House QC?
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Sure. At any rate (get it? RATE??) La Chatlierās principal, if followed to the T, would mean if you put enough CO2 pressure above it that youāll recarboxylate. You and I both know that is not the case. Decarboxylation is not considered isentropic aka adiabatic reversible in the canonical sense that La Chatlierās kind of relies upon.
Reaction dynamics are not simple, and perhaps your observation is valid. I donāt think youāre outright incorrect or lacking in understanding of the subject. But either way, I donāt think weāre going to fully define the subject weāre discussing here and now. It would take quite a rigorous kinetic study that ultimately isnt really worth anyoneās time unless weāre trying to publish.
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the vacuum part surprises me more than the pressure tbh.
I stand corrected.
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Vacuum will more quickly rid of your residual solvent and any low boilers that are absorbing collisions that could otherwise provide the required oomf for the molecular discombobulation we refer to as decarboxylation.
Least thatās my take on what the chemists and physicists haz tried to teach me 
ā¦but if enzymes only lower the barrier, and this rxn works in high pressure CO2ā¦
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Iām ready to be proven wrong, I just want to acknowledge the complexity of a true kinetic study of what weāre describing here. I think weāre all thinking of/saying similar things just slightly differently, weāre splitting hairs a little and ultimately this work needs to be done empirically in lieu of a kinetic study (i.e. run a bunch of conditions and just be happy with what conditions work best even though the mechanism is obfuscated).
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Being proven wrong in science means you get new science (or learn new science). I love that shit.
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~starts tying off arm~
āIām here for the 20 CCs of āscienceā please!ā
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I wonder what happened to op? Not much for answering questions I guess 
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Heās swimming around in his Scrooge McDuckian vault of doubloons.
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We were seriously interested in this and before we could talk any numbers and ROI details he was on a plane to sell it for a significant amount to someone else and that we needed to jump on it asap which wasnāt happenimg without the details and proof, then shot us some emails asking if we were still interested a day or so laterā¦
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Iām still very interested in the process and science and Iām not easily taken away by peopleās sense of urgency.
However, I do understand the desire to capture opportunities and am patient to see this tech further grow.
If you wanna buy it I wonāt stop you bro, just saying Iām not going to throw any money at it personally since
1.Iām confident I could recreate a working SOP before the end of summer
2. Iām not confident in what production costs and time are compared to our current costs/time which are INSANELY low already
3.havenāt seen any proof or details from The OP that I would trust sending them anything
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Redesign the protein to fit your compound of choice. Make sure it fits in the correct orientation of the active site. Custom Order a plasmid vector with the protein, Make and purify protein. Then test it. If it works expand your culture and start making protein.
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I appreciate your ability to hunt and smarts
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That is
Step 1 enzyme discovery- selection and design , enzyme screening , this step aims to find the best working enzyme
Step 2 enzyme engineering/ evolution , usually to creat mutations which makes the enzyme work times faster.
Many people can reach step 1 , while Step two is usually the hardest part.
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Step two is where clever selection schemes beat the hell out of screening.
Raise your hand (say; turn blue or glow) if you can do what I want vs drop dead if you canāt.
Going from ānot quite deadā to āthrivingā is then mostly about time and mutagenesis.
It is certainly possible for the determined & talented to model much of the way there these daysā¦
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Exactly, and AI helps a lot on protein engineering too nowadays, my former workmates are able to complete various projects in a much shorter period
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