Hi guys,
currently planning my medium make up and wondered if anybody has used L-ascorbic acid as an antioxidant before. I know the use is debated and so it would be greatly appreciated if you have experience using it in cannabis shoot tip culture. The purpose of my TC is to multiply plants as fast as possible from micro shoots in order to create clones for commercial operations.
My media mix till now consists of:
- Full strength ms medium incl Vitamin matrix
- TDZ/NAA depending on which phase
- DMSO as an organic solvent (no need to sterilize stocks when using it)
- a buffer to keep the ph stable
If you have any additions or something I forgot pls tell.
Thanks a lot in advance