Whats up everyone, thought it would be fun to spark conversation with a chromatogram I have. The sample tested is distilled mother liquor. There are a couple peaks that would be neat if someone could identify or speculate about.
Peak #24 I’m 99% sure is CBE from comparing 3rd party results (i still need an inhouse standard)
Peak #40 I’m 99% sure is CBT same situation
Peak #26 I’ve got no idea, I’ve see it in other samples, but not in this quantity.
Peak CBNA (doubled peak in green) its obviously not CBNA despite my software’s integration
Peak CBCA its obviously not CBCA since this mother liquor has been distilled before and after isolation
I’ve seen d10 misidentified as CBC more than once with DADs, but that doesn’t specifically answer the CBCA question. Getting MS data would be helpful but doesn’t seem to be in the cards here.
Not sure which software you’re using, but to clean up the integration of the baseline, look around in your integration settings for a “Area Peak Reject” option and bump that value up.
Thanks for your input, Ive definitely heard the same thing about cbc/d10 but cbca is a little odd.
As far as “area peak reject” I know exactly what youre talking about, Ive used it in Xcalibur Thermo Fisher software, but Im using Gilson software currently, and the settings are extremely limited
The following is the only integration settings I can find, and im trying to find a sweet spot where it doesnt integrate the noise, but I still get all the peaks I want… hahaha
Don’t have much experience with the Thermo software. Sorry. I’ve got an old Agilent 6890 and my favorite options for integration is this window. Peak rejection gives me great glee.
Its 50mg sample into 6.66mL, then a 1:40 dilution. I usually run a low for minors, a high for cbd. Its just worked the best for me with this equipment. To give you an idea, that THC peak is 5.11%
The cbna peak could be cbr (cannabiripsol), I’ve seen some literature that puts the peak there. Also the cbca area seems to have a lot of minors that show up in my experience, have not been able to nail them down sadly. Could be C3 flavors of god knows what. But if the material was heat treated, especially over clays or whatever, probably d10.
If you can set your DAD to collect 200-400nm, might be worth a rerun so you can have more data to ID on. There are some minors which have signature uv absorbance. 228 is solid for quantitation and all, but it’s good to get the full picture for ID… Gives you that “magnify and enhance” experience.
