for those that can’t, it can be done for closer to $5k if you’re keen like this guy
shared by @mushroom.miraculum over here: Grain of Choice? - #25 by mushroom.miraculum
CHAPTERS
0:00 Bioprospecting Marine Fungi
0:47 Creating Fungi Isolate
1:37 What is DNA Barcoding?
3:36 Step 1: DNA Extraction
5:08 Preparing 0.5M NaOH Lysis Buffer
6:30 Creating TBE Buffer Stock Solution
7:26 Testing pH
10:34 Marine Fungi Culture Sample
11:39 Aspergillus Fungi Culture
14:56 Notes on PCR Thermocyclers
15:42 Step 2: DNA Amplification (PCR)
15:48 ITS Primers
23:43 Step 3: Gel Electrophoresis
24:02 Creating Gel Running Buffer
26:04 Casting Agarose Gel
31:46 Loading the Gel with PCR Product
34:07 DNA Ladder Reference
34:25 Running the Gel
35:37 Thanks!