Premium CBD Labs out of Madison, WI offers full profile potency testing (11 cannabinoids) for $50/sample with a 2-day turnaround on results. Each sample is run in duplicate (twice) to ensure accuracy and consistency via HPLC. I don’t know any other lab running samples twice just to make sure the results match.
Shoot me an email if you want more info: jherman@premiumcbdlabs.com
we run samples twice… or sometimes in triplicate. Its pretty standard in CO to do
Well it is good to know someone else is doing that too. I haven’t talked to any labs yet that do. Which lab are you with? How big of a sample do you require?
The boss move here is to have your own equipment, test in house, and then send off for third party to see the variance across the two.
Sure, in the perfect world and if you have 100K+ for proper equipment…and a lab director.
Testing your own analytical tools against other ones is probably the only way you’re ever going to know if you’re accurate or not. It’s a good practice to send off occasional samples to verify your own results.
Only reason I would bring it up is because I could do it if I had the funding.
It’s standard protocol to run three tests on each sample and average them and determine the variance and standard deviation. I believe my company sent you a sample, we never paid anything… but your results were very far off from what I found on my own GC. Also the CoA was very ambiguous and looked like a GC was used and not an HPLC like you claim.
Hey Davidb, are you sure you sent to us? When did you send? I think you have the wrong company. We have only been open since August and use HPLC. Not to mention we will be using our own proprietary technology that no other lab has come 2020. If you want more info on that, please shoot me an email. Have you ever heard of MALDI-TOF?
I think most people would haha.
I just confirmed it was in fact your company. This happened last week. I will upload the CoA that was sent once it’s forwarded over to me, it’s on my colleagues email and they’re picking up dry ice at the moment.
Yes, please do. Sorry you feel the results are “ambiguous.” We have actually had a ton of clients come back and say they prefer our report over others because it is very simple, straight forward and easy to read but to each their own. In regards to HPLC vs GC, we 100% use HPLC to test and will soon use MALDI-TOF which no other lab has.
I believe you about the HPLC, but on the CoA you should be explicit and say exactly what machine and apparatus was used.
And what purpose does a MALDI-TOF have in quantifying cannabinoids? Those are great in biochemistry and for identifying macromolecules, I’ve used one as a crude way to identify microorganisms, (each microbe has a unique spectra) and its a lot cheaper than sequencing the dna.
A few things here Davidb. First off, of course your test results will be different if you are using a GC and we are using HPLC. I can give you the model number of the HPLC we are running. Also, how did you prep the samples? What temp did you bake at? Which extraction did you use? Which solution are you using? I could ask 100 questions about how you ran your testing VS how we ran ours. GC are also expensive as hell haha. You have one in house? When was the last time it was calibrated? MALDI-TOF is a more robust version of mass spec. You can get a lot better resolution, lower limits of detection and you can run 300 samples an hour. In regards to quantifying cannabinoids? This one should be obvious if you know about testing and technology…I can explain if needed.
Yes I have an SRI GC in house and it was actually very inexpensive. I understand they’re are a lot of different variables, but 20%+ of my cbd shouldn’t just disappear, no matter what analytical equipment you use. I’ve sent the same sample to another lab and will update the results.
Good on you for being the first to implement a MALDI-TOF into the analytical side. I am actually curious how exactly it works for quantifying cannabinoids if you don’t mind explaining. I’ve only used one a few times for a very different purpose, several years ago.
300 samples an hour? How exactly does it do 5 samples every minute? That must be a scary looking auto-sampler 
Here’s the CoA:
Data_20190927_2019 263_192660011RR2.PDF (350.8 KB)
Hmmm, all the GC I have seen that are legit are 80K+. But anyways, can you answer these questions for me.
300 samples an hour, explained: It’s plate based (96-300+ samples per plate) with a sample taking about 10 seconds each to run.
Next time, you could also reach out to the lab and discuss your concerns with them before hopping on a public forum. Appreciate the conversation, give us a ring if you want to discuss further! Looking forward to your answers 
We also ran your test 3 times…forgot to add that.
Maldi tof doesn’t pop to mind when talking cannabinoids, there isn’t really a need for a matrix and laser to desorb cannabinoids. They go into gas phase just fine when injecting dissolved cannabinoids in organic solvent. On the other hand I bet you can get a super fancy maldi unit used cheap since not many people use them anymore.
Also not sure why GC versus hplc would give a different number except for acidic cannabinoids and you can do an extra step and test for those with GC so that’s actually a crock of shit to claim that they’d be different
As far as all the calibration points, yes that’s all good things to discuss in one of these analytical chemistry pissing contests.
Green house growing system
triplicate is generally the analytical standard - what do you do when those two numbers are different?