I think they’re polymers because it is a plant product, and it makes sense.
I understand all that second part. Hence why I’m asking here. But whatever. Just delete the thread, you all are clearly incapable of simply engaging on a topic. your past has jaded you to everything.
i am trying to determine conformation of complex molecules with multiple stereocenters though.
No, youre not. You have no idea what youre looking for.
Also, roiplek is probably one of the top 5 chemists on this fourm as far as ability and background goes. Your ignorance is insane. Most people dont like his arbasiveness and dont give him credit for that alone.
Are you saying you have no idea what you’re crystallizing?
Or are you under the impression you can get usable X-ray or NMR data from complex mixtures?
Can you list the general possibilities? (Oligo-nucleotides, poly-saccharides, poly-peptides…).
How many of those are testable hypotheses?
You need to understand that you are over your head in complexity issues. Plus you are being vague . Have you thought about crowd sourcing on Reddit?
Whoever hired you and shared their NDA info with you may have hired the wrong person for this particular task.
Grok is rather competent in the field of chemistry…you might try bouncing ideas there as well. Start with the “phenomenology of dynamic crystallization from aqueous protic induced by temperature increase”…I will leave the exercise to you.
Regards.
If you say so. It’s not so much his abrasiveness I take issue with, its his hypocrisy.
I know exactly what I’m crystallizing, it is a molecule with over 50 atoms with 2 stereocenters.
It is the starting material I haven’t had the chance to do exploratory testing on to determine the polymers that are preventing crystallization.
The possibility is a polysaccaride, most likely amlyose or amlyopectin, but also could be some sort of flavonoid or secondary metabolite. I get that I’m being kind of vague, but I’ve seen people be 100% more vague, not cause of any NDA but just because they assume the people they’re talking to can deduce what they mean from very little information, and people, including myself, not take issue with it and merely humbly try to meet them where they are at. It’s like I’ve found a group of people chomping at the bit to criticize the people around them. I haven’t been rude, demeaning, arrogant, or anything in this thread that everyone else has been. I haven’t misused words or engaged in pseudo scientific explanations or anything else that I have been accused of in this thread either. If you have a bunch of analytical chemists in here who have shirked human contact long enough to learn how to run their own instruments and now are spiteful towards the human race, well that’s what you signed up for when you became an analytical chemist. Chill the fuck out. God I hate chemists.
I understand all of this and yet here we are and nothing is going to change. But yea, the person who hired me, we are doing just fine. You don’t have to understand something from first principles to use it successfully. I came here looking for resources I haven’t yet been able to find to try to understand those first principles better. Something everyone should be doing, I think you all think I’m trying to solve a problem. The problem has been solved. I’m trying to better understand the solution.
So not much need for nmr or X-ray crystallography there…
At least not initially.
My point is that neither NMR nor crystallography will get you the information you need here (least that’s my take on those techniques, never having performed them…).
You’re welcome to (mis)interpret my comments as rude or nasty or any other way you see fit. I’m just trying to explore the problem as presented.
Testable hypothesis: Iodine?!?
Sure…one could also argue don’t know what’s IN your solution and that unknown is getting in your way…
Edit: our all knowing overlords suggest that 2D NMR can in fact provide usable data from complex mixtures…
Solution NMR for complex mixtures" refers to using Nuclear Magnetic Resonance (NMR) spectroscopy in liquid solution to analyze mixtures containing many different chemical compounds, where the complex nature arises from overlapping signals in the spectrum due to similar chemical structures within the mixture, making identification and quantification of individual components challenging; however, advanced NMR techniques like 2D correlation experiments can help separate and identify components within a complex mixture.
Still believe @anon45638961 has it right
Yea, it’s not an immediate need, it’s something I’ve had in the back of my mind to determine what contaminants/adulterants/other shit is in my stuff to see what is stopping that crystallization, or if I’m just at a super saturation point at a certain temp that those partially soluble compounds are more soluble in, making the alkaloid less soluble, but I don’t think that’s the case because it’s not a biphasic system, either way I’m sure I’m completely wrong about all of this.
But you can 100% make that argument. I literally made that argument already so you don’t have to tip-toe around saying that, however most people here thought that since I don’t know what the non target molecules in solution, they took it as I don’t know what I’m crystallizing. Either way, I was wrong. About what, I don’t know exactly, but I was wrong, and I’m false. But from my understanding, which was from reading Clayden’s book (Organic chemistry : Clayden, Jonathan : Free Download, Borrow, and Streaming : Internet Archive) that you for unknown solutions, to exactly determine what is in solution, you would need mass spec/NMR/IR combined to make that determination. Maybe that’s what you meant by 2D NMR. But I’m sure since it’s me saying it, I’m completely wrong. So I’ll just work from that perspective for now.
But yea, I’m looking for TLC and flash column right now, I finally have the budget to get those. So yea I’m going to grab them, but my current situation is that.
But yea you’ve been fine or whatnot, but pretty much you and like two other people in this thread haven’t lost their minds completely over the title of this thread. It’s wild.
Whoopee! Are you done?
When you are finished, write up a paper, have it reviewed, accepted and published. Send us the DOI…we will be able to criticize it in a more memorable way.
Criticize deez nuts
you are tarnishing my reputation as an insufferable, aggressive cunt. stop it at once
Not to mention active and prolific pornhub video commenter.
