Hi @danielfp. I just read your good blog post, Are Iron chelates of humic/fulvic acids better or worse than synthetics? Excellent material as always!
I have a few questions, and I thought it would be helpful to post them here for a wider audience of dicot growers. And because this is a long message, the formatting is easier to read here.
You wrote:
An additional concern is the oxidation state of the Fe. While Fe chelates are usually prepared using ferrous iron (Fe2+), these iron chelates are quickly oxidized in solution to their ferric iron (Fe3+) counterparts, especially when the solution is aerated to maintain high levels of oxygen. Since Fe3+ is both more tightly bound to chelates and more reactive when free ā so more toxic when taken up without reduction ā plants can have an even harder time mining Fe3+ out of chelates
Please correct me if Iām wrong, but from what I have read, Fe(o,o-EDDHA) is prepared with Fe(III), not Fe(II), and the same for Fe(DTPA) and Fe(EDTA); at least for Dissolvine (E, D, and Q) and Sprint. Are you aware of manufacturers that prepare Fe(o,o-EDDHA) or Fe(DTPA) using Fe(II) for horticulture?
I reviewed your references, and I didnāt see anything showing an aerated nutrient solution affecting Fe(EDDHA) chelates or the form of iron in the chelate. I may have missed something, though. Can you refer me to research showing aeration of a nutrient solution affects the form of iron (II vs. III) within Fe(EDDHA) chelate? I am especially interested to learn of research on DO2 supersaturation using nanobubbles.
As one of your references showed, it appears dicots preferentially uptake the meso isomer compared to the d,l-racemic mixture. In contrast, monocots use forms equally well. And that the chelation strength of the two forms may be the issue, as the d,l-racemic mixture is 500-times more stable than the meso isomer. For example, pepper showed zero to minimal uptake of the d,l-racemic mixture, and tomato showed a significant preference for the meso isomer. According to AlcaƱiz et al. (2005), āIn Strategy I, Fe(o,o-EDDHA) must enter the root apoplast to get in contact with the reductase on the cell membrane. Part of the chelating agent is then temporally retained in the free space of the apoplast and again turned back to the nutrient solution. It would explain the oscillation observed in Fig. 5.ā
Due to preferential uptake of the meso isomer by dicots and slow transformation of the racemic form into the meso form, it appears the expected equilibria of d,l-racemic and meso forms is not maintained in hydroponic solutions for dicots. So, a greater concentration of the racemic form is expected in recirculating solutions after some time. Which can lead to iron deficiency in recirculating hydroponics from the limited uptake of the racemic form and slow transformation of the racemic form into the meso form.
According to CerdĆ”n et al., (2006), āThe results suggest that the use of Fe(o,o-EDDHA) products with a higher percentage of meso isomer could be more efficient, at least in hydroponics for strategy I plants, than the use of the present products containing 50% of each isomer.ā
Do you think Fe(EDDHA) with higher meso isomer ratio would be benefical for cannabis? And are you aware of a commercial or research source of Fe(o,o-EDDHA) with a higher percentage of meso isomer? Itās possible to separate the racemic and meso from a solution of Fe(o,o-EDDHA) where they are in equilibrium, but for growers that doesnāt seem feasible.
The use of humic/fulvic acids complimented with either unchelated Fe or with some lower proportion of stronger iron chelates, seems to be a better overall choice in terms of both plant uptake and economic expense. As shown by several studies mentioned in this post, the effect of humic/fulvic acids and synthetic chelates might actually be synergistic, with both providing different advantages that can be complimentary in hydroponic solutions. These humic/fulvic acid solutions might also be much more favorable for monocot species, where the use of highly stable Fe(EDDHA) chelating agents does not cure deficiency symptoms.
What concentration ranges of fulvic acid in solution or relative to Fe(EDDHA) would you expect to provide the described synergistic effect?