Degumming enzyme in ethanol?

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Recently got some degumming enzyme from Best Value Vacs. Had a hard time stirring the hydrated enzyme into distillate + water without letting the temperature go above 40C. It was suggested that I can degum by dissolving distillate in a bit of ethanol and adding the hydrated enzyme to this and stirring with stir bar at 40C. Before I try this, I just wanted to get some feedback here. I figured that ethanol would denature the enzyme rendering it useless. Is this not the case? I believe the SOP mentions not exposing the enzyme to ethanol. Anyone else successfully degum in ethanol?

Also, I plan to try Carbon Chems degumming clay soon which sounds like an easier process, but for now I just have the enzyme powder.

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Bump.

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Get the enzyme dissolved in water first, warm the solution and keep well mixed.

Add it to the warm ethanol-oleoresin mixture.

Will it denature? That’s a possibility, but I think that (considering the polarity of EtOH and/or MeOH) it wouldn’t denature the enzymes entirely, like a strong acid would.

A lot of the structural components of enzymes (and all proteins) have to do with hydrogen-bonding, and van der waals forces, and ionic functional groups.

That being said, EtOH doesn’t seem “strong” enough, to denature the enzyme immediately, but as reaction time increases, maybe?

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