I do not chill it at all usually except to room temp. I used to and even used a dry ice bath for a while but it didn’t seem to remove any more waxes than just room temp runs so I don’t do that anymore. I cheat a little by prewetting the alumina column with water and it really slams the brakes on the waxes which load up on top.
Water doesn’t seem to alter the target compound during the boil or afterward in a way that I can detect. Purging the water is an art form lolz and can be a pain but I do it on each monthly med run and am getting practiced at it.
I am not sure what the question about testing means? I do not understand the terms. This Is part of my SOP because it saves me a whole run through the sublimation rig at least. I just refine for myself and small quantities I generally just vape it afterward as far as testing. The sublimator picks up the green if present and it mixes with the THC. It actually has a nastalgic flavor that way kind of but my goal is pale yellow because that is where I enjoy this medicine the most.