Cannabis Tissue Culture

Botany and Cultivation Tissue Culture
41

I think this tissue culture is really interesting. It is surprising that some have had some difficulty getting things off the ground, I was under the impression that the science is teachable.

42

I found the biggest issue to be contamination and the need for a sterile environment.

I ordered a bunch of stuff from this place about 8 years ago and did a bunch of experiments.

http://www.kitchenculturekit.com/

I was using a ghetto glove box, and had lots of contamination issues.

I bought all the parts to build a laminar flow hood, so when I get a chance I plan on giving it another tryā€¦I think Iā€™ve had the parts for over a yearā€¦so Iā€™m not exactly in a rush.

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43

Hi Cyclopath
I am very interested in tissue culture. Did you ever find a gig? Any links or help on where to find out as much as I can about TC would be so appreciated. I am interested in larger scale production, approximately 50,000 a month.

44

The lab Iā€™m currently attached to has a tissue culture division thatā€™s just getting set up now. Excited to see how it goes.

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45

Donā€™t get your hopes up too high. Itā€™s hard and many have failed. Some also have figured some things out but the economics donā€™t support the effort.

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46

There is a book called ā€œPlants from test tubesā€ that covers the topic. Itā€™s a little old, but full of info.

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47

I looked into TC and your budget is what determins if you will be successful or not. The leaders are the Israeliā€™s in this feild.

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48

My personal preference for tissue culture is in saving genetics after some sort of disaster, be it weather, pest, or pathogen. Iā€™ve known too many growers that lost genetics but could have saved them with a tissue culture.

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49

Roots squash you be looking for
It s a complicated process but ok
Revege your precious and either feminine the seeds

50

I would never do it in house, but if one of my mothers came down with mosaic id definitely pay for culture

51

Iā€™d like to create a breeding library. I donā€™t really want to keep males around, so TC would be a nice state to keep them In until they are needed.

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52

I bought a tissue culture kit a few months back. In Hopeā€™s to keep sterile strains for extended periods of time for a genetic bank.
I havenā€™t ran it yet. I have a few seeds I plan on starting in it. Should be getting around to it a few weeks.

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53

Alan is the man! Heā€™s killing it in the fungi world too. Iā€™ve known him for years, what a great guy.

54

Not necessarily true, at least not 4 years ago when I saw someone sink a ton of money into it, paying a phd scientist and full build out of tc lab, for a 100k Sqft green house for it to be out done by regular clones in every way.

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55

When people imagine TC in there head for commercial purposes unless they have seen it in RL they have no clue. A 100k greenhouse is barely what you need to grow out some mature plants. Serious commercial GH structures start in the 500k range. Great videoā€¦

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56

If anyone does TC on here I have a couple epic strains Iā€™d like brought back to OG status.

Dm

You are allowed to keep each pheno I send as well.

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57

Wouldnā€™t peace of mind introducing KNOWN clean clones going into a mass production grow and the ability to keep genetics in a test tube be worth whatever (well obviously there is a ceiling) tc costed?

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58

I realize this post is 2 years old, but it seems tc is taking off on a larger scale. However many still go with tried and true aero cloners

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59

Any updates?

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60

You probably know Phinest (TC)

https://phinestcannabis.com/

@phinestcannabis IG

Serious canna porn;

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