Photo sequence of a DCVC run. The color bands are well known to me and the crude always seperates out in this sequence. One photo is image enhanced to highlight bands. These were just archived lab notes pix I took but thought this would be a good start for a topic.
This is what a DCVC sep looks like, time stamped with starting dewaxed dark brown crude testing nominally at 60% THC. Test tubes only show fractions up to but excluding the green band. Dark brown test tubes have target compound. Evap…
I have fielded many questions about chromatography and one I get is just how to calculate the depth of the column to use. This tip is unorthodox and likely not in chemistry texts but it has suited me very well. Once done you really do not need to do it again.
DCVC works by ramping up the polarity of the mobile phase in a continuous gradient. Each addition to the mobile phase is made more polar and so as it washes the compound through the stationary phase then each addition of say 20 ml of solve…
This started as dark brown concentrate testing 65% nominal cannabinoid. The crude was dissolved into hexane and then wet loaded on top of a used column. I load it right on top of the Celite 545 layer (Amazon) from a dry loaded run. The Celite layer works GREAT when wet loading a bunch. This run started at one ounce. The column handled it with zero problems. This run was not dewaxed or processed at all prior to this first run.
The solvent system is generally Ethyl Acetate and Hexane starting wit…
“The solvent system is generally Ethyl Acetate and Hexane starting with hexane alone. Then the Ethyl Acetate is added in 5% increments and is collected 20ml fractions at a time. Once the gradient is over about 30% Ethyl Acetate to hexane the majority of wanted fractions have eluted. Then I add Ethyl Acetate alone along with methanol to move the waxes and chlorophyls and just general gunk off the column.”
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