Hello all
i have some material with the following Chromatogram. Posting to see if anyone has a cannabinoid elution order that can be used to identify what the possible unknowns are. Any help would be greatly appreciated, thanks.
3 Likes
Can tell ya the shoulder on the left of your delta 9 peak is almost certainly exo-thc
Can you repost that jpg. Image …with a sharper image it would be easier to read.
If you’re on a pc can double click the image and it zooms quite a bit
1 Like
Restek HPLC 16pt Std…pdf (88.8 KB)
2 Likes
Steve: it would seem to me that you are running a decarbed marijuana (not hemp) mixture on a RP18, water to organic gradient, with either ACN or MEOH as B phase with a little formic acid as pH modifier.
Since we really have no idea of the rate of gradient production or whether your separation is isocratic (notice the chromatogram posted below has such details appended). So you can sort of compare if you have some thing similar …but it would help if you mentioned your details of separation.
Your machine does note the position of some neutrals…but the overall chromatogram sort of suggests you need some adjustments ….
Show us a chromatogram of standards…and your question is about a vague as one could get?
smoke some of that. You should get high…when you burn it in your pipe…you can add about 2-3 hundred extra unknowns going into your lungs.
3 Likes
How could we possibly answer this question when you didn’t post what method you used? Let’s start there.
1 Like
Like others have said sharing your method, a run of your calibrants, and if you really wanna know what your shoulders and what not are I’d recommend MS if that’s an option.
But you’re gonna wanna separate out those shoulders from your stronger peaks to have a good chance in reliably quantifying