A/B Extraction and Isolation of Psilocybin

First person I believe who has mentioned ultrasonic in thread if I’m correct. I’ve been curious about the yield for ultrasonic after seeing the ice cube tek on fresh harvest material and then seeing an ultrasonic manufacturer claiming a cold water extraction.

The ice cube tek in general reminds me of some tek folllowing the dead for tea that I saw used on fresh for margaritas but alas Ill go way off topic boomer style talking about that further…

Also curious if anyone has done any 300-400nm blue light testing on mycelium as its been a reddit craze trying to use blue light for pinning and higher yield. While I’m doubtful fruit bodies would actually benefit the fact that actives don’t show up in mycelium until exposed to light makes me wonder if while the fruit bodies aren’t necessarily effected the mycelium may very well benefit.

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big hold up with sonication for me is scalability. sounds like you have a solid process.

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I’m sure a jewelry sonicator would work fine as long as you grind up your material.

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lol didn’t realize you could buy 30 L ones. disregard my last comment.

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I’ve been helping a buddy of mine with Cordy extract.

The extraction Tek works pretty well across species. gonna try some LM next.


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Alcohol/water I presume?

That color seems to be common among fungus extracts, do u think it’s the trehalose or some kind of pigment?

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Lion’s mane is likely gonna be yellow.

cordyceps just happen to be that orange color.

the psilo extract when done from albinos is more of a gold color than orange.

seems like coincidence that it happens to be the same color. I’m leaning towards it being a pigment though. as I can get clear and colorless trehalose to crash on its own.

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DiNKLB3RG how have you been dehydrating your extractions? vacuum dessicated in the rotovap or vacuum dessicater? Are the big rocks dephosphorlated in acetic acid?

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ive dried with the roto, I’ve dried in a vac chamber.

no

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I’ve never gotten it to become that dry in the rotovap. Most of the methanol carriers over at 25°c and the last residual methanol is driven off at 32°c max. This leaves an extract thicker than thc distillate and once cool is firm but malleable.
How are you getting it so dry? Time,temp?
Which method of desiccation do you prefer.

Have you considered that maybe his extract isn’t drier than yours, maybe it’s got more impurities that give it more structure than your methanol extracts

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Different solvents, different polarities, different problems, different impurities.

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That happen to be from the MycoSymbiotics team?

I tried extracting w 190proof ecerclear a bunch of the trimming of the stalks…got nothing but a grey substance that stinks of shrooms. I let It soak for free days before filtering and attempting to dewax

@DiNKLB3RG what’s I do wrong

still got moisture in your sugar structure…

I’ll do methanol today if the shop has it in.

James specifically

try a shorter warm soak…then filter as it comes to room temp. the gray is likely fats+starches that precipitate out. they are often the impurities left behind in the roto. see pics

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Mine didn’t have any yellow stuff…I guess I need to try a hot extraction next time

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DiNKLB3RG, what are you using as a non-polar solvent in the sep. funnel?

sep funnel is just the 170-190 proof extract. no NP. wanted to see how it separated at room temp bc of the wax/lipid content.

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…And then?

How is it defatted / dewaxed? Are you allowed to talk yet? Big fan of your work and pics.

I’ve only gotten as far as the quote, then dumped everything into gummies.

“Smells like mushrooms still” gummies.

Edit: thanks @DiNKLB3RG . I’m using 180 proof reflux distilled corn etoh, not exactly lab grade but fun for testing.

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mechanical separation of precipitated insolubles. I decanted and saved the bottom layer. tossed the top 2, and proceeded with evaporation.

could probably toss it in the fridge and get more to drop out.

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