Cool, thanks! Do you know if that number holds true for heptane denatured ethanol too? I’m wondering if the addition of heptane (in which lipids are quite soluble) requires even lower temps to avoid lipid extraction.
The reason I asked, is that normally my extractions don’t warm up past -60C. Last one got as warm as -50C and resulted in cloudy distillate. See post here:
So I’m wondering if I extracted more lipids than normal at the slightly higher extraction temp of -50C (in heptane denatured ethanol). Or if the cloudiness is from not doing a good job of pulling off the heads before swapping flasks during the distillation.