it would be useful to overlay the chromatogams. which is one reason developing a good working relationship with your 3rd party lab is so important. especially if you don’t yet have In House analytics
There are two possibilities, you’re pulling to fast, and not getting separation, so you’re pulling over the same ballast that you pulled the first time. Or you’re running too hot, and actually degrading your cannabinoids rather than purifying them.
You could tell these apart yourself if you had a GC or HPLC. with a test on your leavings, and an obliging 3rd party lab, you might also be able to figure it out.
note: “too fast” is essentially equivalent to “too hot” and both are related to vac depth.