Method development. How to split co-eluting peaks?

I’ll admit, I’m more well versed in liquid chromatography, but I think I’ve dabbled enough in GC to get your wheels turning.

There are a few variables you can start with:

  1. injection volume
  2. Carrier gas type
  3. Carrier gas flow rate
  4. Column type - I’m not sure what your column is made of, but Restek makes a reliable fused silica column for RS. Obviously if your analytes are not attracted to the stationary phase, you won’t get separation
  5. Oven temperature/ramp rate

Here is a good link from Restek showing great separation between pentane and acetone:
https://www.restek.com/pdfs/solventsincannabis_csc-poster.pdf

Check it out and let me know if you are still having trouble. I have a hunch it’s going to come down to your oven parameters. Maybe try something along the lines of what Restek has, I think starting at 140C is a bit hot for the volatile compounds.

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