Reading it now. Im quoting some stuff here I find really useful for those of us with machines who can run these material tests.
"3.2.3 Thin Layer Chromatography (TLC)
Samples were manually spotted on 10x20 cm reversed phase (C18) silica gel plates F254 No.
105559 (Merck, Darmstadt, Germany) and developed in saturated normal chambers
(saturation time 15 minutes). Eluent was methanol : 5% acetic acid, 19:1 (v/v). After
development, visual inspection was done under UV 254nm. General visualization of
compounds was done by spraying with modified anisaldehyde-sulphuric acid spray reagent
[Stahl, 1967]. For selective visualization of cannabinoids the TLC plate was sprayed with 0.5%
Chapter 3
42
fast blue B salt (Sigma) in water, followed by 0.1M NaOH [Corrigan, 1980]. Reference
standards were used for identification of chromatographic spots. "