Chromatography Units for CBD Isolation

Hash and Stuff Pre/Post-Processing
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Damn, that’s badass! What would one of these run me?? Just out of curiosity.

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not sure to be honest its custom.

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Did you buy this for thc removal from crude or to make isolate?

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THC removal

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Here is an IG short vid of my small scale efforts using standard phase DCVC running a solvent tradient over silica gel 60.
https://www.instagram.com/p/BoWNJEDFQlJ/

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Thanks for sharing this man, this is cool. I followed you on IG too.
What solvents did you use for your mobile phase, if you don’t mind me asking? And what did you start with, distillate?

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I always use Ethyl Acetate and Hexane as my mobile phase. This was some first run disty if memory serves. First run without distillation first will reveal a lot of green following the red brown cannabinoid colors but the cannabinoids definately overlap into the green band as well.

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Have you tried regular phase? Ive been able to get seperation via tlc and small columns. Cheaper and can re-use if you really need to.

Obviously cannot reuse as much as reverse phase but you can reuse a bit.

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@ZizzleB These threads may also be helpful for your pursuits. I’m in a similar boat trying to find a more easily scaleable method than chromatography for removing THC from hemp extract, so anything useful I find in experimenting with these methods mentioned I’ll be happy to share.

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Ah, thank you for this! I am going to dig through these now and I will also do the same, in reporting any useful info I find. :metal:

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I can help you with developing a method to pack this! I will likely need to get a spec sheet break down, or come there to check the build of it out in person. Do you have any solvent pumps yet? Isocratic or something like that? Or vessels to mix solvent ratios in?

What are you trying to achieve here? Seperation of d9 thc from the CBD? Or just purification of a crude oil?

Dm me and I’ll shoot you over my rates and a # to call.

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You’ll need a TON of c18 media, which can be used over 100 times if cleaned between runs.

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If you want a great deal on media(Si60, C18, etc) for that thing hit me up.

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Have you ever used fumed silica or precipitated silica? I have a ton of samples laying around that I don’t have any use for at the moment.

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Havent used them! Idk what their benefits may be, over standard silica.

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Novasep Makes a pretty good continuous and its freaking brilliant, it automatically ratifies and pulls off. NOVASEP | Services and technologies for the life science and chemical industries - Novasep

Here is a cool video explaining its awesomeness.

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imageI want one of these systems with the solvent recycling system. I haven’t heard back from them for a quote. I’m wondering if they think I can’t afford a million dollar setup. Sure, something worth below $100/g…but what if you don’t just run Cannabis…?

Any takers? (I’m only half kidding) There’s a lot of cheap, terrible material from Asia that can be bought and purified and resold. Pictured above is an example of an item I developed. Profit ~$250/g. 1 step synthesis from commercially available materials. I could start making it tomorrow in my lab with just one tank of argon and some supplies from emerald scientific. But I digress…the people who make Cannabis oil are medicinal chemist as far as I’m concerned.

Does anyone have quotes for the Varicol series? Or a distributor phone number? The one in the US is essentially a CMO/consulting group.

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What’s the best and cheapest supplier of c18 you have found

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I miss you , but I know you are still watching

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Complete newbie Michigan hemp processor, very interested in column dcvc chromatography to remove thc from crude, hope to scale to 200g batches of cbd crude, and currently debugging with a small bench setup, but lots of questions.

  1. I would rather use reverse phase since I can use safer solvents, 85/15 methanol or ethanol in water, but cant afford typical c18 costs for large scales. Seems like old school researchers made their own with paraffin or silicone coated “Kieselgur” which I had to look up, and os D.E. Anyone ever dabble with this? I tried heating freshwater diatomaceous earth to 300f to dry it out and mixee with food grade silicone, and seems very hydrophobic and packs nicely but haven’t run it yet
  2. I struggle with reason for using step vs gradient vs isochratic solvents. If I use isochratic, seems like easier to reuse. Plus, HPLC seems to rely on isochratic in that you arent dipping the plate in multiple solutions. How much is lost and why not use isochratic?
  3. Would prefer wet loading, how viscous is ideal? Am going to try a 10% load to CV ratio, any hints on optimizing max size of load vs cv?
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