Carboxylation - Adding the "a" back to THC

one could certainly be evolved for the task in short order. the enzyme they used looks like it’s target is close enough structurally to use as a starting point.

you amplify the gene (PCR) under low fidelity conditions, and look for clones that decarb our favorite cannabinoids.

I’d wager an enzyme would be the most regio-specifc way to put the carboxyl back too. the best candidate from above might be a good starting point, under different reaction conditions, but screening through the first pool as well makes good sense.

If I could see a good way to make decarbing/REcarbing THC a life saving (and thus selectable) trait, it would be faster.

Edit: …and now we’re officially off topic. thread title is “Chemistry”!!

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