Ultra Performance Chromatography (UPC) Theory and possible application

co2expanedsolventtheory.pdf (123.5 KB)

“The idea of enhancing the mass transfer properties by adding
compressed CO2 to an organic solvent comes from the field of gas
expanded liquids (GXLs), for CO2 called CXLs. In chemical engineering,
GXLs are used in chemical synthesis and material processing
as a rapid way to change the solvent and mass transfer properties
of a liquid solvent [3]. What happens when the solvent is pressurized
with CO2 is that it volumetrically expands, but not necessarily leading
to decreased density. Diffusivity increases, and the
solvent properties change. For instance, when ethanol or methanol
is pressurized with CO2, the polarisability (dielectric constant) decreases.
In chromatography, it is the drastic increase in diffusivity,
which is attractive and is inherently leading to more efficient separations.
A few years ago, chromatography with mixtures of compressed
CO2 and organic solvent as mobile phase in packed column LC has
been developed even further through the launch of Waters Ultra
Performance Convergence Chromatography (UPC2) system, along
with Agilent and Jasco’s similar SFC systems. What has changed is
the view on gradient elution, in which an increase in molar fraction
of co-solvent is applied rather than a change in pressure and/or temperature.”

Im think this could be an easy process to use for cannabis oil on a preparative scale.
This can be done below the 1000 psi meaning sanitary “Stainless Steel Sanitary Clamp – 13MHP” could be used, making construction much easier.
there is also very interesting possibilities like countercurrent/concurrent modes which can be complicated to explain but involve flowing a different ph mobile phase backward at certain points so that u can maximize separation.