I’ve ran a couple experiments with a dry loaded chromatography column. I was doing a modified DCVC setup a la @Beaker and had great success with doing a water/etoh gradient. My problem is my alumina is brown/purple and I can’t actually see anything that goes on. So I’m really just trudging into experiments pretty blindly and interpreting results.
If anyone has a good source for alumina hook me up! (I’m in Canada, but can order from U.S. if needed. I’ve got a couple samples coming in from China for pretty cheap).
When at room temperature everything behaved as to be expected and pulled off the red/yellow gradient to great success. When I run the column with super chilled etoh (-40c) I noticed I was getting the chlorophyll/brown gradients first. Can anyone explain why this would be? I had read that some aspects of etoh polarity changes at low temperatures? Would it reverse the process so all my undesireables get eluted on the first pass?