Cloudpoint Extraction of D9 using Dowfax 20B102

Abstract A cloud point extraction coupled with high performance
liquid chromatography (HPLC/UV) method was
developed for the determination of Δ9-tetrahydrocannabinol
(THC) in micellar phase. The nonionic surfactant “Dowfax
20B102” was used to extract and pre-concentrate THC from
cannabis resin, prior to its determination with a HPLC–UV
system (diode array detector) with isocratic elution. The
parameters and variables affecting the extraction were investigated.
Under optimum conditions (1 wt.% Dowfax
20B102, 1 wt.% Na2SO4, T=318 K, t=30 min), this method
yielded a quite satisfactory recovery rate (~81%). The limit of
detection was 0.04 μgmL−1, and the relative standard deviation
was less than 2 %. Compared with conventional solid–
liquid extraction, this new method avoids the use of volatile
organic solvents, therefore is environmentally safer.cloudpoint.pdf (316.4 KB)

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